Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	151.6 / NA	43	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/25);  total (18/25)

TACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAA  >  NZ_CP009273/49783‑50039                                                                                                                                |                                                                                                                                  tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACg                                                                                                                         >  2:740626/1‑138 (MQ=255)   cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCa                                                                                                                       <  2:844777/138‑1 (MQ=255)   cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCa                                                                                                                       <  1:672893/138‑1 (MQ=255)   cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCa                                                                                                                       <  2:417170/138‑1 (MQ=255)   cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCa                                                                                                                       <  2:115149/138‑1 (MQ=255)   cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCa                                                                                                                       <  2:832413/138‑1 (MQ=255)           ggCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACAACTta                                                                                                               >  2:739848/1‑138 (MQ=255)              gACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaata                                                                                                           <  2:201635/139‑1 (MQ=255)                  aTTTTTTCTATCGGGAAAACTCAATGATCAGTCTGATTTCGGCGTTAGCGGTAGATCGCGTTATCGGCATGAAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc                                                                                                       <  1:357349/139‑1 (MQ=255)                     ttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCg                                                                                                     <  1:640464/138‑1 (MQ=255)                                  aaTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                                                                       <  1:740626/139‑1 (MQ=255)                                        aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                                                                                     <  1:653492/135‑1 (MQ=255)                                         aTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                                                                <  2:611470/139‑1 (MQ=255)                                                gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCCTGTCGTGGAACCTGCCTCCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat                                                                          >  2:711251/1‑138 (MQ=255)                                                gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat                                                                          >  2:168843/1‑138 (MQ=255)                                                 tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGttt                                                                                                                              >  2:743738/1‑85 (MQ=255)                                                 tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGATGTGGAACCTGCCTACCGATCTCGCCGGGttt                                                                                                                              <  1:743738/85‑1 (MQ=255)                                                        gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt                                                                  <  2:846792/138‑1 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAccc                                                                                                      <  1:324755/99‑1 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAccc                                                                                                      >  2:324755/1‑99 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGGTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAAACGggcgtc                                                              >  1:796727/1‑139 (MQ=255)                                                           gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                                                              >  1:208027/1‑139 (MQ=255)                                                                       gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGa                                                   <  1:765337/138‑1 (MQ=255)                                                                            cgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                              >  1:371766/1‑138 (MQ=255)                                                                             gTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaaa                                            <  1:739848/139‑1 (MQ=255)                                                                                 tCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAata                                         <  2:371766/138‑1 (MQ=255)                                                                                               gCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGt                           >  1:727817/1‑138 (MQ=255)                                                                                                   tGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAAcc                      >  1:6025/1‑139 (MQ=255)                                                                                                   tGCTGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAAcc                      >  1:145023/1‑139 (MQ=255)                                                                                                    gctgTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCg                     >  1:109796/4‑139 (MQ=255)                                                                                                    gCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                                                                                      <  1:104962/42‑1 (MQ=255)                                                                                                    gCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                                                                                      >  2:104962/1‑42 (MQ=255)                                                                                                       gTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGt                  <  1:665682/139‑1 (MQ=255)                                                                                                        tGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCaa                        <  2:154933/132‑1 (MQ=255)                                                                                                        tGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCaa                        >  1:154933/1‑132 (MQ=255)                                                                                                          gAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTAc                <  1:264522/138‑1 (MQ=255)                                                                                                          gAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACg               >  1:306576/1‑139 (MQ=255)                                                                                                            aCCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGa             <  2:145023/139‑1 (MQ=255)                                                                                                              ctgcctacCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACg           <  1:361364/139‑1 (MQ=255)                                                                                                               tgcctaccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCAAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACgc          >  2:684305/1‑138 (MQ=255)                                                                                                                        aTCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                              <  2:536349/94‑1 (MQ=255)                                                                                                                        aTCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                              >  1:536349/1‑94 (MQ=255)                                                                                                                             gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTaa  <  1:711251/133‑1 (MQ=255)                                                                                                                                |                                                                                                                                  TACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAA  >  NZ_CP009273/49783‑50039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑