Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,549,065	C→T	A288T (GCG→ACG)	maeA ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,549,065	0	C	T	100.0%	101.0 / NA	28	A288T (GCG→ACG)	maeA	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

TTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGC  >  NZ_CP009273/1548934‑1549198                                                                                                                                    |                                                                                                                                      tttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCtt                                                                                                                                >  2:186279/1‑139 (MQ=255) tttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCtt                                                                                                                                >  1:809677/1‑139 (MQ=255)   tCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCtt                                                                                                                                <  1:186279/137‑1 (MQ=255)   tCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTtgc                                                                                                                              >  2:206613/1‑139 (MQ=255)       ccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCg                                                                                                                          <  1:205973/139‑1 (MQ=255)         gcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGAt                                                                                                                        >  2:23413/1‑139 (MQ=255)                          aaTCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCCCCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGt                                                                                                         <  2:324443/137‑1 (MQ=255)                                 cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgccg                                                                                                 >  1:182720/1‑138 (MQ=255)                                  gcgcTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                               <  2:182720/139‑1 (MQ=255)                                  gcgcTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                               <  1:23413/139‑1 (MQ=255)                                          tCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCt                                                                                        >  1:264528/1‑138 (MQ=255)                                                  atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCAt                                                                               >  1:684163/1‑139 (MQ=255)                                                  atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCAt                                                                               >  2:659254/1‑139 (MQ=255)                                                    gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCg                                                                             >  2:239045/1‑139 (MQ=255)                                                                  tGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAt                                                               >  1:383808/1‑139 (MQ=255)                                                                            cGCTGACCCTGCGCCTAGGAAGAAGATTTTTTTCTCGCTTAACTGACCTCCTGCCGTGCGGCTTGCTGCGATCAGTGTCCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGa                                                     <  2:486966/139‑1 (MQ=255)                                                                            cGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGa                                                     <  2:809677/139‑1 (MQ=255)                                                                              cTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGa                                                     >  1:850045/1‑137 (MQ=255)                                                                                  aCCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCgg                                               <  2:383808/139‑1 (MQ=255)                                                                                   ccTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCATTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGt                                              <  1:321932/139‑1 (MQ=255)                                                                                       cgcCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGt                                                                                                         >  2:233809/1‑76 (MQ=255)                                                                                       cgcCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGt                                                                                                         <  1:233809/76‑1 (MQ=255)                                                                                       cgcCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAg                                          >  1:172393/1‑139 (MQ=255)                                                                                                                gCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCa                 <  1:659254/139‑1 (MQ=255)                                                                                                                 cTTAACTGACCCCCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCaa                <  2:155465/139‑1 (MQ=255)                                                                                                                         accaccTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCtt          <  1:811168/137‑1 (MQ=255)                                                                                                                          ccaccTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa        >  1:847598/1‑138 (MQ=255)                                                                                                                                gCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGc  >  2:464735/1‑138 (MQ=255)                                                                                                                                    |                                                                                                                                      TTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGC  >  NZ_CP009273/1548934‑1549198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑