Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,550,159	C→T	R21H (CGC→CAC)	sra ←	stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,550,159	0	C	T	100.0%	152.2 / NA	39	R21H (CGC→CAC)	sra	stationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/20);  total (19/20)

AAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACG  >  NZ_CP009273/1550026‑1550280                                                                                                                                      |                                                                                                                           aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGGTTTGTCACCTTCGGTAACTATTTTGTGCTgg                                                                                                                       >  1:564569/1‑139 (MQ=255) aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTgg                                                                                                                       >  1:632848/1‑139 (MQ=255)             gcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTa                                                                                                           <  1:148792/139‑1 (MQ=255)                 gCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGCAATTTTATGgt                                                                                                       >  1:646339/1‑139 (MQ=255)                 gCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgt                                                                                                       <  1:64486/139‑1 (MQ=255)                  cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                      <  1:776543/139‑1 (MQ=255)                  cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                      <  1:450848/139‑1 (MQ=255)                  cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                      <  2:381856/139‑1 (MQ=255)                  cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                      <  2:428708/139‑1 (MQ=255)                        tgtTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGGTTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcc                                                                                                <  2:564569/139‑1 (MQ=255)                        tgtTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcc                                                                                                <  1:28181/139‑1 (MQ=255)                          tttAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcca                                                                                               <  1:248102/138‑1 (MQ=255)                           ttAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcc                                                                                                >  2:28181/1‑136 (MQ=255)                           ttAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcc                                                                                                >  1:834962/1‑136 (MQ=255)                                             tGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATg                                                                                      >  2:699669/1‑127 (MQ=255)                                             tGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATg                                                                                      <  1:699669/127‑1 (MQ=255)                                             tGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACg                                                                          >  1:458453/1‑139 (MQ=255)                                             tGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACg                                                                          >  1:4764/1‑139 (MQ=255)                                             tGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACg                                                                          >  1:597169/1‑139 (MQ=255)                                               aTGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcca                                                                                               >  2:150728/1‑117 (MQ=255)                                               aTGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcca                                                                                               <  1:150728/117‑1 (MQ=255)                                               aTGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGt                                                                        >  1:687466/1‑139 (MQ=255)                                                    tATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGAt                                                                   >  2:677128/1‑139 (MQ=255)                                                            aCTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCAttt                                                            >  2:788685/1‑138 (MQ=255)                                                             cTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCAtttt                                                           >  2:535211/1‑138 (MQ=255)                                                                ttCAGCGGGGCTTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                                         <  2:196767/137‑1 (MQ=255)                                                                   aGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAActcct                                                    <  2:4764/139‑1 (MQ=255)                                                                   aGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAActcct                                                    <  1:788685/139‑1 (MQ=255)                                                                                       ttGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGtttt                                 <  2:646339/139‑1 (MQ=255)                                                                                          ggTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAg                              <  2:687466/138‑1 (MQ=255)                                                                                                      cGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATTGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTa                                <  2:570206/124‑1 (MQ=255)                                                                                                      cGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTa                                >  1:570206/1‑124 (MQ=255)                                                                                                      cGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCg                 >  1:310428/1‑139 (MQ=255)                                                                                                         tGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccag                                                                                                   <  1:410116/55‑1 (MQ=255)                                                                                                         tGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccag                                                                                                   >  2:410116/1‑55 (MQ=255)                                                                                                                   aCCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAaca    <  1:677128/139‑1 (MQ=255)                                                                                                                     cTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                                         <  2:302071/84‑1 (MQ=255)                                                                                                                     cTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                                         >  1:302071/1‑84 (MQ=255)                                                                                                                     cTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACg  >  1:205706/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                           AAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAG‑AATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACG  >  NZ_CP009273/1550026‑1550280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑