Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,603,097	A→C	G61G (GGT→GGG)	yneE ←	bestrophin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,603,097	0	A	C	100.0%	103.8 / NA	31	G61G (GGT→GGG)	yneE	bestrophin family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/23);  total (8/23)

ATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NZ_CP009273/1602962‑1603226                                                                                                                                        |                                                                                                                                  aTCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGAcccc                                                                                                                                 >  2:121004/1‑138 (MQ=255) aTCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCA‑TATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCga                                                                                                                               <  1:828137/139‑1 (MQ=255)  tCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCga                                                                                                                               <  2:338060/139‑1 (MQ=255)    cGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCgaga                                                                                                                             <  2:401512/139‑1 (MQ=255)                               tAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTg                                                                                                  <  1:121004/139‑1 (MQ=255)                                      cGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCa                                                                                           <  2:432814/139‑1 (MQ=255)                                       gTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGcc                                                                                             >  1:562663/1‑136 (MQ=255)                                       gTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGcc                                                                                             >  1:750287/1‑136 (MQ=255)                                         gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGAt                                                                                        >  1:694204/1‑139 (MQ=255)                                          aGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGAt                                                                                        <  2:468157/138‑1 (MQ=255)                                            gCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  2:694204/139‑1 (MQ=255)                                                          cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGCCCCCGAGAATGCTGaa                                                                                                                     <  2:485107/93‑1 (MQ=255)                                                          cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGaa                                                                                                                     >  1:485107/1‑93 (MQ=255)                                                          cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     >  2:80406/1‑125 (MQ=255)                                                          cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  1:80406/125‑1 (MQ=255)                                                          cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcagc                                                                       <  2:296853/139‑1 (MQ=255)                                                          cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcag                                                                        >  1:296853/1‑138 (MQ=255)                                                                                     aCGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAgaga                                            <  1:640430/139‑1 (MQ=255)                                                                                                       attaCGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAgcg                           <  1:638521/138‑1 (MQ=255)                                                                                                       attaCGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAgcg                           <  2:758127/138‑1 (MQ=255)                                                                                                           cGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAgg                      <  2:223716/139‑1 (MQ=255)                                                                                                             aaaaCCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     >  1:488273/1‑74 (MQ=255)                                                                                                             aaaaCCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  2:488273/74‑1 (MQ=255)                                                                                                                aCCAAGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:757788/139‑1 (MQ=255)                                                                                                                   aaGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAg                                                                                          <  1:147537/63‑1 (MQ=255)                                                                                                                   aaGAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAg                                                                                          >  2:147537/1‑63 (MQ=255)                                                                                                                     gAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAGTTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:728377/139‑1 (MQ=255)                                                                                                                     gAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:256416/139‑1 (MQ=255)                                                                                                                     gAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:399211/139‑1 (MQ=255)                                                                                                                     gAAAAATGGCGATGGCGACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:68203/139‑1 (MQ=255)                                                                                                                          atggcgatggcgACCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGataata       <  2:503983/139‑1 (MQ=255)                                                                                                                               gatggcgaCCCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAg  <  2:750287/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  ATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NZ_CP009273/1602962‑1603226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑