Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,635,187	T→C	P42P (CCT→CCC)	ynfU →	putative zinc‑binding protein YnfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,635,187	0	T	C	100.0%	109.3 / NA	29	P42P (CCT→CCC)	ynfU	putative zinc‑binding protein YnfU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

AGGAAAAGCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGA  >  NZ_CP009273/1635051‑1635316                                                                                                                                         |                                                                                                                                  aGGAAAAGCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCa                                                                                                                                 >  1:676859/1‑139 (MQ=255)   gAAAAGCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCAtt                                                                                                                               >  1:345638/1‑139 (MQ=255)        gCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGcaa                                                                                                                           >  1:598417/1‑138 (MQ=255)             tGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTg                                                                                                                              >  2:672704/1‑130 (MQ=255)             tGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTg                                                                                                                              <  1:672704/130‑1 (MQ=255)             tGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACaaa                                                                                                                       <  1:255937/137‑1 (MQ=255)                      gTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGAc                                                                                                            <  2:598417/139‑1 (MQ=255)                       tAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACa                                                                                                           <  2:676859/139‑1 (MQ=255)                        aaaaaCTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACa                                                                                                           >  2:142644/1‑138 (MQ=255)                                aaaaTCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                    <  2:345638/137‑1 (MQ=255)                                aaaaGCACGCCGTAATTAGCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTGTCAAGAGTACAAAAAGGTGCCCTTTTGATCGGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                    <  2:343253/137‑1 (MQ=255)                                            tAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCtga                                                                                      <  1:660672/139‑1 (MQ=255)                                                      gTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTgattgatt                                                                                <  1:506393/135‑1 (MQ=255)                                                      gTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTttat                                                                            >  2:432763/1‑139 (MQ=255)                                                       ttAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTttatt                                                                           <  1:109093/139‑1 (MQ=255)                                                       ttAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTttat                                                                            >  2:289943/1‑138 (MQ=255)                                                          aaTGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGt                                                                        <  1:636505/139‑1 (MQ=255)                                                          aaTGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGt                                                                        <  2:562145/139‑1 (MQ=255)                                                                                     agTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTaataat                                             <  2:421528/139‑1 (MQ=255)                                                                                                aGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAg                                   >  1:272856/1‑138 (MQ=255)                                                                                                                      gTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACa                                                                                                           >  1:376333/1‑44 (MQ=255)                                                                                                                      gTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACa                                                                                                           <  2:376333/44‑1 (MQ=255)                                                                                                                       tGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGt                                                                        >  1:512020/1‑78 (MQ=255)                                                                                                                       tGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGt                                                                        <  2:512020/78‑1 (MQ=255)                                                                                                                       tGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAg           <  1:150601/139‑1 (MQ=255)                                                                                                                         cccTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCg         >  2:112615/1‑139 (MQ=255)                                                                                                                              ttGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGttt    >  2:539423/1‑139 (MQ=255)                                                                                                                                 aTCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGttt    >  2:715344/1‑136 (MQ=255)                                                                                                                                 aTCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTga  <  2:132443/138‑1 (MQ=255)                                                                                                                                      ccccCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                    <  2:569262/35‑1 (MQ=255)                                                                                                                                      ccccCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                    >  1:569262/1‑35 (MQ=255)                                                                                                                                      ccccCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGtt     >  1:689211/1‑130 (MQ=255)                                                                                                                                      ccccCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGtt     <  2:689211/130‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  AGGAAAAGCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGA  >  NZ_CP009273/1635051‑1635316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑