Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,718,009	A→G	E544E (GAA→GAG)	ydhK →	FUSC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,718,009	0	A	G	100.0%	147.6 / NA	40	E544E (GAA→GAG)	ydhK	FUSC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

CGTCGCGTTGGCGTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAATTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTGCGC  >  NZ_CP009273/1717874‑1718135                                                                                                                                        |                                                                                                                               cGTCGCGTTGGCGTGGTTAGCGTTCGCCTTTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGttt                                                                                                                             <  2:748693/139‑1 (MQ=255)     gcgTTGGCGTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGaa                                                                                                                          <  1:643911/138‑1 (MQ=255)             gTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACt                                                                                                                 <  1:181630/139‑1 (MQ=255)                     cGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTAtcatca                                                                                                         >  2:103381/1‑139 (MQ=255)                       ttCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACg                                                                                                       <  1:20042/139‑1 (MQ=255)                             aTTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACgtcagtc                                                                                                 <  2:704022/139‑1 (MQ=255)                               tCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGtcagtcag                                                                                               >  2:30529/1‑139 (MQ=255)                                     tCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGt                                                                                         <  1:821369/139‑1 (MQ=255)                                             cGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGc                                                                                   >  1:276158/1‑137 (MQ=255)                                               gATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAgg                                                                               <  2:381935/139‑1 (MQ=255)                                               gATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAg                                                                                >  2:65116/1‑138 (MQ=255)                                               gATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAg                                                                                >  1:100026/1‑138 (MQ=255)                                               gATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAg                                                                                >  1:703628/1‑138 (MQ=255)                                                    tCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATgcg                                                                          >  2:668470/1‑139 (MQ=255)                                                          aaGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCt                                                                    <  1:103381/139‑1 (MQ=255)                                                               gccgccATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGCGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCg                                                               <  2:615178/139‑1 (MQ=255)                                                                ccgccATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGt                                                              <  1:711742/139‑1 (MQ=255)                                                                 cgccATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGtt                                                             <  1:37240/139‑1 (MQ=255)                                                                           gcgcgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACg                                                   <  1:65116/139‑1 (MQ=255)                                                                              cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGttatta                                                     <  1:356857/134‑1 (MQ=255)                                                                              cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGttatta                                                     >  2:356857/1‑134 (MQ=255)                                                                              cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                <  1:668470/139‑1 (MQ=255)                                                                              cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                <  1:684807/139‑1 (MQ=255)                                                                                cTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGtta                                                        >  2:488305/1‑129 (MQ=255)                                                                                cTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGtta                                                        <  1:488305/129‑1 (MQ=255)                                                                                      cGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAg                                        <  2:780263/139‑1 (MQ=255)                                                                                                tCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGaa                              <  2:297122/139‑1 (MQ=255)                                                                                                tCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGaa                              <  2:100026/139‑1 (MQ=255)                                                                                                   aTCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTg                           <  1:30529/139‑1 (MQ=255)                                                                                                          aaGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCAt                    >  1:246081/1‑139 (MQ=255)                                                                                                                 cATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCt             >  1:693151/1‑139 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAg                                        <  1:555065/100‑1 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAg                                        >  2:555065/1‑100 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGtg                                      <  1:379107/102‑1 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGtg                                      >  2:379107/1‑102 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  2:852299/1‑138 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  2:64413/1‑138 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  1:704742/1‑138 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  1:665625/1‑138 (MQ=255)                                                                                                                             aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  1:362352/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                               CGTCGCGTTGGCGTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAATTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTGCGC  >  NZ_CP009273/1717874‑1718135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑