Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,744,804 T→C E156G (GAG→GGG)  ydhX ← 4Fe‑4S dicluster domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,744,8040TC97.5% 138.3 / ‑2.7 40coding (467/669 nt)ydhX4Fe‑4S dicluster domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (16/23);  minor base . (1/0);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-01

GGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATC  >  NZ_CP009273/1744669‑1744926
                                                                                                                                       |                                                                                                                          
ggTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAg                                                                                                                         >  1:785152/1‑139 (MQ=255)
    gCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCa                                                                                                                     <  1:498859/139‑1 (MQ=255)
         tatTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAAt                                                                                                                <  1:53549/139‑1 (MQ=255)
         tatTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAAt                                                                                                                <  1:456925/139‑1 (MQ=255)
          atTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAAt                                                                                                                >  1:771719/1‑138 (MQ=255)
          atTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAAt                                                                                                                >  2:190139/1‑138 (MQ=255)
           ttGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAg                                                                                                                    >  2:520045/1‑133 (MQ=255)
           ttGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAg                                                                                                                    <  1:520045/133‑1 (MQ=255)
                    tttatttTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGAC‑CAGCGCAGAAATCACATTTATCTg                                                                                                    >  1:440057/1‑139 (MQ=255)
                       atttTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGc                                                                                                   >  2:130071/1‑138 (MQ=255)
                        ttttGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGcc                                                                                                  <  1:276669/138‑1 (MQ=255)
                         tttGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCAc                                                                                                >  1:407876/1‑139 (MQ=255)
                             ctgcAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACtt                                                                                              >  2:600851/1‑137 (MQ=255)
                                cAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGt                                                                                          >  1:153215/1‑138 (MQ=255)
                                 aaCCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGa                                                                                        <  1:383632/139‑1 (MQ=255)
                                 aaCCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGa                                                                                        <  1:7950/139‑1 (MQ=255)
                                 aaCCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGa                                                                                        <  2:500296/139‑1 (MQ=255)
                                  aCCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGAc                                                                                       <  1:434223/139‑1 (MQ=255)
                                  aCCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGAc                                                                                       <  1:130071/139‑1 (MQ=255)
                                  aCCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGAc                                                                                       <  1:626235/139‑1 (MQ=255)
                                            aaTTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAg                                                                              <  2:785152/138‑1 (MQ=255)
                                                 cTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGc                                                                         <  1:495418/138‑1 (MQ=255)
                                                    ggCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAAc                                                                     <  2:153215/139‑1 (MQ=255)
                                                     gCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAAcc                                                                    <  2:212134/139‑1 (MQ=255)
                                                         tCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTgg                                                                 >  1:298410/1‑138 (MQ=255)
                                                              gcgACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACAttt                                                                                                         <  2:333536/93‑1 (MQ=255)
                                                              gcgACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACAttt                                                                                                         >  1:333536/1‑93 (MQ=255)
                                                                            aTGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGc                                              >  2:775472/1‑138 (MQ=255)
                                                                             tGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCaa                                            >  1:784562/1‑139 (MQ=255)
                                                                               cGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAAt                                           <  1:686177/138‑1 (MQ=255)
                                                                                   ttCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTg                                      >  1:268713/1‑139 (MQ=255)
                                                                                     ccGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGc                                     <  2:771719/138‑1 (MQ=255)
                                                                                     ccGGGCAGGCGATGACGCAAATGGGAGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTAATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACGGc                                     <  1:235816/138‑1 (MQ=255)
                                                                                        ggCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGAc                                                                                       <  2:222110/85‑1 (MQ=255)
                                                                                        ggCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGAc                                                                                       >  1:222110/1‑85 (MQ=255)
                                                                                              cgcTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGt                                                                                  <  1:59440/84‑1 (MQ=255)
                                                                                              cgcTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGt                                                                                  >  2:59440/1‑84 (MQ=255)
                                                                                                                         ttGCCAGTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATc  <  2:407876/137‑1 (MQ=255)
                                                                                                                               gTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGa                                                                                        >  1:409609/1‑45 (MQ=255)
                                                                                                                               gTCGGGACCCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGa                                                                                        <  2:409609/45‑1 (MQ=255)
                                                                                                                                       |                                                                                                                          
GGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATC  >  NZ_CP009273/1744669‑1744926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑