Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,820,976	C→T	A69T (GCG→ACG)	astE ←	succinylglutamate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,820,976	0	C	T	100.0%	184.3 / NA	47	A69T (GCG→ACG)	astE	succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/29);  total (18/29)

CCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAA  >  NZ_CP009273/1820851‑1821110                                                                                                                              |                                                                                                                                       ccAACGCCCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAAcgcg                                                                                                                           <  2:368049/139‑1 (MQ=255)     cGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCa                                                                                                                       <  2:377022/139‑1 (MQ=255)      gACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAg                                                                                                                      <  1:551041/139‑1 (MQ=255)              aaCATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCca                                                                                                              <  1:650035/139‑1 (MQ=255)              aaCATCCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATAGCGTGCCAAGCAACGCGTCCAGCATCTCca                                                                                                              <  1:751260/139‑1 (MQ=255)                     gATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                                                                                                       <  1:119874/139‑1 (MQ=255)                     gATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                                                                                                       <  1:634400/139‑1 (MQ=255)                     gATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                                                                                                       <  1:236149/139‑1 (MQ=255)                       ttCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCg                                                                                                     <  2:667355/139‑1 (MQ=255)                       ttCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCg                                                                                                     <  1:835273/139‑1 (MQ=255)                          atatCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtct                                                                                                  <  1:72642/139‑1 (MQ=255)                           tatCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCg                                                                                                     >  2:824209/1‑135 (MQ=255)                           tatCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCg                                                                                                     <  1:824209/135‑1 (MQ=255)                             tCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                                                                                                <  1:36939/138‑1 (MQ=255)                               gCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTa                                                                                             <  1:145025/139‑1 (MQ=255)                                 tATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                                                                                                       >  2:107779/1‑127 (MQ=255)                                 tATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                                                                                                       <  1:107779/127‑1 (MQ=255)                                 tATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAcc                                                                                           >  1:785672/1‑139 (MQ=255)                                   tGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAcc                                                                                           <  2:676567/137‑1 (MQ=255)                                    ggCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtg                                                                                        >  1:404516/1‑139 (MQ=255)                                                      gCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCAc                                                                      >  1:675813/1‑139 (MQ=255)                                                           aGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGt                                                                  <  1:306393/138‑1 (MQ=255)                                                           aGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGt                                                                  <  2:159784/138‑1 (MQ=255)                                                             cgcAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTgcg                                                               <  1:403072/139‑1 (MQ=255)                                                                     gaTTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCgg                                                       <  1:676586/139‑1 (MQ=255)                                                                      aTTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGt                                                      <  2:785672/139‑1 (MQ=255)                                                                       ttCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgt                                                                                         >  2:536980/1‑103 (MQ=255)                                                                       ttCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgt                                                                                         <  1:536980/103‑1 (MQ=255)                                                                       ttCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTg                                                     >  2:220927/1‑139 (MQ=255)                                                                          ccGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTg                                                                 <  2:196597/124‑1 (MQ=255)                                                                          ccGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTg                                                                 >  1:196597/1‑124 (MQ=255)                                                                          ccGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCg                                                  >  2:748680/1‑139 (MQ=255)                                                                            gAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCt                                                                              <  2:37577/109‑1 (MQ=255)                                                                            gAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCt                                                                              >  1:37577/1‑109 (MQ=255)                                                                                        cAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGt                                    >  1:458917/1‑139 (MQ=255)                                                                                          aCCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTc                                  >  2:703078/1‑139 (MQ=255)                                                                                            cGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTc                                  >  1:346217/1‑137 (MQ=255)                                                                                            cGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTc                                  >  2:444533/1‑137 (MQ=255)                                                                                             gCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAg                               <  1:579567/139‑1 (MQ=255)                                                                                               cAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGca                             <  2:404516/139‑1 (MQ=255)                                                                                               cAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGca                             >  2:98650/1‑139 (MQ=255)                                                                                                   gTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACAcc                         <  1:398566/139‑1 (MQ=255)                                                                                                     aaCGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                                                                                                       >  2:198351/1‑59 (MQ=255)                                                                                                     aaCGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                                                                                                       <  1:198351/59‑1 (MQ=255)                                                                                                     aaCGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCAt                       >  2:344689/1‑139 (MQ=255)                                                                                                                    tGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAgccagcgc         >  1:5792/1‑138 (MQ=255)                                                                                                                           tCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGaa  <  2:675813/138‑1 (MQ=255)                                                                                                                              |                                                                                                                                       CCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAA  >  NZ_CP009273/1820851‑1821110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑