Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,891,283	A→G	D32G (GAT→GGT)	sdaA →	L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,891,283	0	A	G	100.0%	230.2 / NA	63	D32G (GAT→GGT)	sdaA	L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/30);  total (33/30)

GTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGG  >  NZ_CP009273/1891151‑1891403                                                                                                                                     |                                                                                                                         gTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGt                                                                                                                    >  1:392492/1‑139 (MQ=255) gTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGt                                                                                                                    >  2:50823/1‑139 (MQ=255)           gTCCAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGc                                                                                                          >  1:423554/1‑139 (MQ=255)              cAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTa                                                                                                       <  2:826542/139‑1 (MQ=255)                             gAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACt                                                                                        >  2:389960/1‑139 (MQ=255)                                  ttatCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGt                                                                                   >  2:837979/1‑139 (MQ=255)                                  ttatCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGt                                                                                   <  1:275334/139‑1 (MQ=255)                                   tatCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTAc                                                                                                      <  2:81050/119‑1 (MQ=255)                                   tatCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTAc                                                                                                      >  1:81050/1‑119 (MQ=255)                                        tGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGt                                                                             >  2:394194/1‑139 (MQ=255)                                          aTTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            >  2:709580/1‑138 (MQ=255)                                          aTTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            >  2:535849/1‑138 (MQ=255)                                          aTTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            >  2:297790/1‑138 (MQ=255)                                             aGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGAc                                                                         <  1:389960/138‑1 (MQ=255)                                               tCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCt                                                                                                         >  2:601344/1‑104 (MQ=255)                                               tCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCt                                                                                                         <  1:601344/104‑1 (MQ=255)                                                   ttCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGttt                                                                     <  2:246111/136‑1 (MQ=255)                                                    tCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATgg                                                                 >  2:233663/1‑139 (MQ=255)                                                      gACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGtt                                                               <  2:363912/139‑1 (MQ=255)                                                      gACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGt                                                                <  1:394194/138‑1 (MQ=255)                                                         aTGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCAc                                                            >  1:44426/1‑139 (MQ=255)                                                                 ggTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTg                                                    <  1:50823/139‑1 (MQ=255)                                                                     gggATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            >  1:261229/1‑111 (MQ=255)                                                                     gggATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            <  2:261229/111‑1 (MQ=255)                                                                      ggATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCt                                                     <  1:48807/133‑1 (MQ=255)                                                                      ggATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCt                                                     >  2:48807/1‑133 (MQ=255)                                                                           ggTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTaaa                                           <  1:660862/138‑1 (MQ=255)                                                                           ggTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAg                                          >  2:790175/1‑139 (MQ=255)                                                                             tCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            >  1:303921/1‑103 (MQ=255)                                                                             tCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            <  2:303921/103‑1 (MQ=255)                                                                             tCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGc                                        <  2:44426/139‑1 (MQ=255)                                                                             tCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGc                                        >  2:447333/1‑139 (MQ=255)                                                                             tCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGc                                        <  1:837979/139‑1 (MQ=255)                                                                               ccTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGcca                                      <  1:297790/139‑1 (MQ=255)                                                                                       tCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACAcc                               >  2:497805/1‑138 (MQ=255)                                                                                        cccATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCacac                                <  2:423554/136‑1 (MQ=255)                                                                                        cccATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGa                             >  1:459640/1‑139 (MQ=255)                                                                                                gTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCa                     <  2:235325/139‑1 (MQ=255)                                                                                                gTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTACCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCa                     <  2:459640/139‑1 (MQ=255)                                                                                                 tAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGt                                                                                   <  1:623692/76‑1 (MQ=255)                                                                                                 tAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGt                                                                                   >  2:623692/1‑76 (MQ=255)                                                                                                  aGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAg                                                                                                            <  2:647083/50‑1 (MQ=255)                                                                                                  aGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAg                                                                                                            >  1:647083/1‑50 (MQ=255)                                                                                                  aGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGatat                          <  1:445842/132‑1 (MQ=255)                                                                                                  aGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGatat                          >  2:445842/1‑132 (MQ=255)                                                                                                  aGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCat                    >  2:277214/1‑138 (MQ=255)                                                                                                  aGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCat                    >  1:794922/1‑138 (MQ=255)                                                                                                     gCCTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCattatt                <  2:392492/139‑1 (MQ=255)                                                                                                      ccTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            <  2:323084/78‑1 (MQ=255)                                                                                                      ccTATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCAttatta               >  1:34134/1‑139 (MQ=255)                                                                                                      ccTATGAAGACAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTg                                                                            >  1:323084/1‑78 (MQ=255)                                                                                                        tATGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATg             >  1:1521/1‑139 (MQ=255)                                                                                                         aTGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACcaca                                 >  1:736317/1‑118 (MQ=255)                                                                                                         aTGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACcaca                                 <  2:736317/118‑1 (MQ=255)                                                                                                         aTGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCattatt                <  1:345487/135‑1 (MQ=255)                                                                                                         aTGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCattatt                >  2:345487/1‑135 (MQ=255)                                                                                                         aTGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCa                     <  2:491962/130‑1 (MQ=255)                                                                                                         aTGAAGGCAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCa                     >  1:491962/1‑130 (MQ=255)                                                                                                                cAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATgg            >  2:593860/1‑132 (MQ=255)                                                                                                                cAGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATgg            <  1:593860/132‑1 (MQ=255)                                                                                                                 aGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCa    <  2:794922/139‑1 (MQ=255)                                                                                                                 aGGTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCa    <  1:447333/139‑1 (MQ=255)                                                                                                                   gTAAACAGTTCGTCGATGGTCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAgg  >  1:527476/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                         GTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGG  >  NZ_CP009273/1891151‑1891403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑