Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,913,346	T→G	V527G (GTG→GGG)	yebT →	lipid‑binding membrane homeostasis protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,913,346	0	T	G	100.0%	148.0 / NA	40	V527G (GTG→GGG)	yebT	lipid‑binding membrane homeostasis protein YebT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/23);  total (17/23)

TTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCA  >  NZ_CP009273/1913214‑1913474                                                                                                                                     |                                                                                                                                 ttCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc                                                                                                                            <  2:30864/139‑1 (MQ=255) ttCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc                                                                                                                            <  1:437970/139‑1 (MQ=255)      ccGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGcaggcagg                                                                                                                         <  1:720860/137‑1 (MQ=255)      ccGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGAt                                                                                                                       <  2:455549/139‑1 (MQ=255)      ccGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGAt                                                                                                                       >  1:227244/1‑139 (MQ=255)         ggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCgg                                                                                                                    >  2:246091/1‑139 (MQ=255)         ggCGATAAAGGGGAGATGAAAGCCAGCTATCCACTGTATTCCCATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCgg                                                                                                                    >  1:610785/1‑139 (MQ=255)              tAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTg                                                                                                               <  1:174015/139‑1 (MQ=255)                      agaTGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCg                                                                                                       <  2:213330/139‑1 (MQ=255)                      agaTGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCg                                                                                                       >  2:181389/1‑139 (MQ=255)                      agaTGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCg                                                                                                       >  1:572668/1‑139 (MQ=255)                       gaTGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCg                                                                                                       >  2:762976/1‑138 (MQ=255)                               ccAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGa                                                                                              <  2:139793/139‑1 (MQ=255)                                        cACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGa                                                                                     <  1:805076/139‑1 (MQ=255)                                             tATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGa                                                                                >  2:155121/1‑139 (MQ=255)                                                gCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTa                                                                             >  2:290890/1‑139 (MQ=255)                                                     tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGAt                                                                                                                       <  1:143035/92‑1 (MQ=255)                                                     tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGAt                                                                                                                       >  2:143035/1‑92 (MQ=255)                                                     tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTg                                                                                                               <  2:109700/100‑1 (MQ=255)                                                     tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTg                                                                                                               >  1:109700/1‑100 (MQ=255)                                                     tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTg                                                                        >  2:215388/1‑139 (MQ=255)                                                     tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTg                                                                        <  1:290890/139‑1 (MQ=255)                                                                         aCAGCCTTAGCGAGTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGGAAAGGGGAAGGTGGTGAAGTGAGTACCGTGCGTCCGCGAGCTAACGCGtt                                                    <  2:610785/139‑1 (MQ=255)                                                                                     aTTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCt                                        <  1:155121/139‑1 (MQ=255)                                                                                      tttACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTg                                       <  1:762976/139‑1 (MQ=255)                                                                                       ttACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGa                                                                                     >  2:351234/1‑92 (MQ=255)                                                                                       ttACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGa                                                                                     <  1:351234/92‑1 (MQ=255)                                                                                            caccacAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATAtt                                 <  2:572668/139‑1 (MQ=255)                                                                                               cacaACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGt                                                                                       <  1:570383/82‑1 (MQ=255)                                                                                               cacaACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGt                                                                                       >  2:570383/1‑82 (MQ=255)                                                                                               cacaACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAg                              >  2:153424/1‑139 (MQ=255)                                                                                                       tGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTa                      <  2:464373/139‑1 (MQ=255)                                                                                                       tGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTa                      >  2:696531/1‑139 (MQ=255)                                                                                                         aGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATc                    >  1:790323/1‑139 (MQ=255)                                                                                                               aGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAAcc              <  1:500622/139‑1 (MQ=255)                                                                                                                      agaCGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGAc       <  1:432144/139‑1 (MQ=255)                                                                                                                      agaCGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGAc       <  1:446050/139‑1 (MQ=255)                                                                                                                           cTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGtt                                                    <  1:59433/89‑1 (MQ=255)                                                                                                                           cTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGtt                                                    >  2:59433/1‑89 (MQ=255)                                                                                                                           cTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCa  <  2:227244/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 TTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCA  >  NZ_CP009273/1913214‑1913474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑