Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,927,446	A→G	V472V (GTT→GTC)	edd ←	phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,927,446	0	A	G	100.0%	212.7 / NA	59	V472V (GTT→GTC)	edd	phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/31);  total (28/31)

AAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCC  >  NZ_CP009273/1927316‑1927580                                                                                                                                   |                                                                                                                                       aaGCGCCGGAGAGTAGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGacac                                                                                                                                 <  1:236614/138‑1 (MQ=255)     gcCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATcg                                                                                                                            <  1:732301/139‑1 (MQ=255)                 tcCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGAATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCaaa                                                                                                                >  1:175355/1‑139 (MQ=255)                   cATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAAcc                                                                                                              <  2:35608/139‑1 (MQ=255)                        gTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCtt                                                                                                         >  1:822734/1‑139 (MQ=255)                         taactaacGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCgg                                                                                                                         >  1:34766/1‑122 (MQ=255)                         taactaacGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCgg                                                                                                                         <  2:34766/122‑1 (MQ=255)                               acGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGc                                                                                                  <  2:431069/139‑1 (MQ=255)                                    aTTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCa                                                                                             <  2:212978/139‑1 (MQ=255)                                    aTTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCa                                                                                             <  2:216379/139‑1 (MQ=255)                                               ccGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGCCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGc                                                                                  >  1:188368/1‑139 (MQ=255)                                                             cacCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCaaaaa                                                                     >  2:45383/1‑138 (MQ=255)                                                             cacCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCaaaaa                                                                     >  1:229480/1‑138 (MQ=255)                                                             cacCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCaaaaa                                                                     >  2:354459/1‑138 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCaa                                                                                                                 >  2:786419/1‑89 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCaa                                                                                                                 <  1:786419/89‑1 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGc                                                                                                  >  2:339555/1‑104 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGc                                                                                                  <  1:339555/104‑1 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCg                                                               >  2:331694/1‑139 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCg                                                               >  1:825558/1‑139 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCg                                                               >  1:18304/1‑139 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCg                                                               >  1:690169/1‑139 (MQ=255)                                                                  aGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCg                                                               >  1:676775/1‑139 (MQ=255)                                                                    tGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCt                                                             >  2:259202/1‑139 (MQ=255)                                                                    tGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCt                                                             <  2:676775/139‑1 (MQ=255)                                                                     ggcggcATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAAcc                                                                                                              >  2:116895/1‑89 (MQ=255)                                                                     ggcggcATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAAcc                                                                                                              <  1:116895/89‑1 (MQ=255)                                                                        ggcATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGgcg                                                         >  1:486087/1‑139 (MQ=255)                                                                         gcATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGgcgc                                                        <  1:45383/139‑1 (MQ=255)                                                                            tGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCtt                                                      >  1:539114/1‑138 (MQ=255)                                                                                    tGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCAcc                                              <  1:46635/138‑1 (MQ=255)                                                                                    tGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCt                                             >  2:20969/1‑139 (MQ=255)                                                                                    tGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCt                                             >  2:411908/1‑139 (MQ=255)                                                                                        aTTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGt                                          <  1:21878/138‑1 (MQ=255)                                                                                         ttCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTg                                                            <  2:147632/119‑1 (MQ=255)                                                                                         ttCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTg                                                            >  1:147632/1‑119 (MQ=255)                                                                                         ttCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTtc                                        >  1:816636/1‑139 (MQ=255)                                                                                         ttCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTtc                                        >  2:431124/1‑139 (MQ=255)                                                                                              gCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAAc                                   >  1:558503/1‑139 (MQ=255)                                                                                              gCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAAc                                   <  1:411908/139‑1 (MQ=255)                                                                                                      ttCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATcg                                                                                                                            <  2:566562/42‑1 (MQ=255)                                                                                                      ttCGCTTTTGGCCCCTGATGACGGACAACGACGACACAATcg                                                                                                                            >  1:566562/1‑42 (MQ=255)                                                                                                             ttGGCCCCTGATGACGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCagag                      <  1:431124/137‑1 (MQ=255)                                                                                                                     tgatgaCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCAcc                                              <  1:100086/105‑1 (MQ=255)                                                                                                                     tgatgaCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCAcc                                              >  2:100086/1‑105 (MQ=255)                                                                                                                     tgatgaCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATaa            >  2:81247/1‑139 (MQ=255)                                                                                                                        tgaCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCg         <  1:36690/139‑1 (MQ=255)                                                                                                                        tgaCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCg         <  2:355309/139‑1 (MQ=255)                                                                                                                          aCGGACAACGCCGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCCCGGCAGAGGTTTTCATAACCGCa       <  1:315744/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  1:259202/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  1:33364/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  2:486087/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  1:629547/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  1:411670/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  2:690169/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  2:822734/139‑1 (MQ=255)                                                                                                                          aCGGACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  2:825558/139‑1 (MQ=255)                                                                                                                            ggACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       >  2:550006/1‑137 (MQ=255)                                                                                                                            ggACAACGACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa       <  1:550006/137‑1 (MQ=255)                                                                                                                               caacgaCGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACTGGCACGGCAGAGGTTTTCATAACCGCACGGcc  <  1:753339/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       AAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCC  >  NZ_CP009273/1927316‑1927580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑