Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,977,975	C→T	E192E (GAG→GAA)	araG ←	arabinose ABC transporter ATP‑binding protein AraG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,977,975	0	C	T	100.0%	203.7 / NA	52	E192E (GAG→GAA)	araG	arabinose ABC transporter ATP‑binding protein AraG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/22);  total (30/22)

GCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCA  >  NZ_CP009273/1977839‑1978106                                                                                                                                         |                                                                                                                                    gCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCttc                                                                                                                                   >  1:188991/1‑139 (MQ=255)  cACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCttct                                                                                                                                  >  2:201572/1‑139 (MQ=255)       gcgcGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg                                                                                                                             <  1:719416/139‑1 (MQ=255)       gcgcGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg                                                                                                                             >  1:664033/1‑139 (MQ=255)       gcgcGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg                                                                                                                             <  1:589489/139‑1 (MQ=255)       gcgcGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg                                                                                                                             <  1:515112/139‑1 (MQ=255)        cgcgTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg                                                                                                                             >  2:62975/1‑138 (MQ=255)          cgtcgtGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAg                                                                                                                          >  1:308193/1‑139 (MQ=255)            tcgtGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGtt                                                                                                                        >  2:232324/1‑139 (MQ=255)                     aCCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATaa                                                                                                               >  2:321317/1‑139 (MQ=255)                           tgcATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCgg                                                                                                          >  2:768316/1‑138 (MQ=255)                                   ggTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGAtt                                                                                                  <  1:321317/138‑1 (MQ=255)                                    gTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATCTTTCTTCCATACGGTGAGAAACGTATAAGATTACCAGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTgc                                                                                                >  2:463081/1‑138 (MQ=255)                                    gTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAAcgc                                                                                                            >  1:64111/1‑127 (MQ=255)                                    gTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAAcgc                                                                                                            <  2:64111/127‑1 (MQ=255)                                       aaGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGa                                                                                             >  2:476136/1‑139 (MQ=255)                                             ttGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg                                                                                                                             <  1:388721/101‑1 (MQ=255)                                             ttGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg                                                                                                                             >  2:388721/1‑101 (MQ=255)                                                aCATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACggg                                                                                    <  2:643500/139‑1 (MQ=255)                                                aCATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACggg                                                                                    <  1:200442/139‑1 (MQ=255)                                                 cATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGc                                                                                   <  1:219583/139‑1 (MQ=255)                                                    aaCGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCa                                                                                  >  2:433513/1‑137 (MQ=255)                                                     aCGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGc                                                                                                                            <  2:744902/94‑1 (MQ=255)                                                     aCGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGc                                                                                                                            >  1:744902/1‑94 (MQ=255)                                                           aTCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAg                                                                         <  1:476136/139‑1 (MQ=255)                                                            tCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGc                                                                        >  1:617455/1‑139 (MQ=255)                                                                   aGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTgg                                                                 <  2:528484/139‑1 (MQ=255)                                                                       aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtca                                                             >  1:256643/1‑139 (MQ=255)                                                                       aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtca                                                             >  2:637519/1‑139 (MQ=255)                                                                       aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtca                                                             >  2:366168/1‑139 (MQ=255)                                                                           aTGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtcatca                                                          <  1:201572/138‑1 (MQ=255)                                                                                 tCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGa                                                   <  1:637519/139‑1 (MQ=255)                                                                                 tCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGa                                                   >  2:37779/1‑139 (MQ=255)                                                                                   gCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTCGGCTCATCAAAGGCGAt                                                  >  2:121306/1‑138 (MQ=255)                                                                                              atatTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGt                                       >  2:75613/1‑138 (MQ=255)                                                                                                               tGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTgg                     >  2:265803/1‑139 (MQ=255)                                                                                                               tGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTgg                     <  2:617455/139‑1 (MQ=255)                                                                                                               tGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTCGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTgg                     >  2:478386/1‑139 (MQ=255)                                                                                                                 agaAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGc                    >  2:532734/1‑138 (MQ=255)                                                                                                                 agaAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGc                    >  2:616196/1‑138 (MQ=255)                                                                                                                 agaAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGc                    >  2:824565/1‑138 (MQ=255)                                                                                                                   aaaCGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATaa                                                                                                               >  2:251442/1‑45 (MQ=255)                                                                                                                   aaaCGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATaa                                                                                                               <  1:251442/45‑1 (MQ=255)                                                                                                                        tataAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCaa            <  1:616196/139‑1 (MQ=255)                                                                                                                        tataAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCaa            <  1:433513/139‑1 (MQ=255)                                                                                                                        tataAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCaa            <  1:265803/139‑1 (MQ=255)                                                                                                                        tataAGAGTACCCGCCCTTCTTGTCGCGGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGTTTGGCTCATCAAAGGCGATAAGTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCaa            <  2:356670/139‑1 (MQ=255)                                                                                                                          taAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTTCGCGCCAGCGCTTTGGCGATTTCAAcc          <  1:121306/139‑1 (MQ=255)                                                                                                                                 aCCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGc    >  1:238052/1‑138 (MQ=255)                                                                                                                                  cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCAt        <  2:108245/133‑1 (MQ=255)                                                                                                                                  cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCAt        >  1:108245/1‑133 (MQ=255)                                                                                                                                  cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCa  >  2:409714/1‑139 (MQ=255)                                                                                                                                  cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCa  >  2:609412/1‑139 (MQ=255)                                                                                                                                  cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCa  >  1:792027/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                    GCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCA  >  NZ_CP009273/1977839‑1978106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑