Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,018,781	A→G	intergenic (+87/‑211)	yodD → / → yedP	peroxide/acid resistance protein YodD/mannosyl‑3‑phosphoglycerate phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,018,781	0	A	G	100.0%	180.7 / NA	48	intergenic (+87/‑211)	yodD/yedP	peroxide/acid resistance protein YodD/mannosyl‑3‑phosphoglycerate phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/32);  total (16/32)

ACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGC  >  NZ_CP009273/2018652‑2018905                                                                                                                                  |                                                                                                                             aCTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGAtg                                                                                                                     <  2:219439/139‑1 (MQ=255)                           ctGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGAtg                                                                                                                     <  1:144814/113‑1 (MQ=255)                           ctGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGAtg                                                                                                                     >  2:144814/1‑113 (MQ=255)                           ctGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAAttttt                                                                                           >  2:635125/1‑139 (MQ=255)                                   aaTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTg                                                                                    >  2:435407/1‑138 (MQ=255)                                     tCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGAc                                                                                  >  2:366883/1‑138 (MQ=255)                                        ttGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGa                                                                              <  1:626470/139‑1 (MQ=255)                                               cacaATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATAtt                                                                       <  2:473212/139‑1 (MQ=255)                                               cacaATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATAtt                                                                       <  1:842220/139‑1 (MQ=255)                                                 caATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCg                                                                     <  1:126783/139‑1 (MQ=255)                                                  aaTAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGAtg                                                                                                                     >  1:433210/1‑90 (MQ=255)                                                  aaTAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGAtg                                                                                                                     <  2:433210/90‑1 (MQ=255)                                                  aaTAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGAt                                                                                                                      <  1:311385/89‑1 (MQ=255)                                                  aaTAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGAt                                                                                                                      >  2:311385/1‑89 (MQ=255)                                                  aaTAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATAtt                                                                       <  2:33791/136‑1 (MQ=255)                                                  aaTAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATAtt                                                                       >  1:33791/1‑136 (MQ=255)                                                   aTAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGcc                                                                   <  2:837178/139‑1 (MQ=255)                                                   aTAAAAAAATCCCGACAATGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTTCACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGcc                                                                   <  2:642200/139‑1 (MQ=255)                                                    tAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCa                                                                  >  1:520715/1‑139 (MQ=255)                                                    tAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCa                                                                  <  1:695761/139‑1 (MQ=255)                                                         aaaTCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATatga                                                             <  2:791968/139‑1 (MQ=255)                                                          aaTCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATatgaa                                                            <  2:79525/139‑1 (MQ=255)                                                              ccGACCCTGAGGGGGTCGGGATGAAACCTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAt                                                        >  1:838075/1‑139 (MQ=255)                                                               cGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                                                       <  2:798060/139‑1 (MQ=255)                                                               cGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATCCGCCATATGAATGAtt                                                       <  2:421724/139‑1 (MQ=255)                                                                 aCCCTGAGGGGGTCGGGATGAAACCTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTaa                                                     <  2:838075/139‑1 (MQ=255)                                                                   ccTGAGGGGGTCGGGATTAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACg                                                   <  1:822335/139‑1 (MQ=255)                                                                    cTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGc                                                  <  1:366883/139‑1 (MQ=255)                                                                           ggTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTAt                                           <  1:32853/139‑1 (MQ=255)                                                                            gTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTAt                                           >  1:647715/1‑138 (MQ=255)                                                                                ggATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCCACTTATGAATa                                      <  1:428334/139‑1 (MQ=255)                                                                                   tGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTAt                                           <  2:284782/131‑1 (MQ=255)                                                                                   tGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTAt                                           >  1:284782/1‑131 (MQ=255)                                                                                     aaaCTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                                                       >  1:722879/1‑117 (MQ=255)                                                                                     aaaCTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                                                       <  2:722879/117‑1 (MQ=255)                                                                                       aCTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGa                                                                              <  2:823235/92‑1 (MQ=255)                                                                                       aCTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGa                                                                              >  1:823235/1‑92 (MQ=255)                                                                                        cTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAAtgtg                              <  1:191737/139‑1 (MQ=255)                                                                                           gCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACg                                                   >  2:58509/1‑115 (MQ=255)                                                                                           gCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACg                                                   <  1:58509/115‑1 (MQ=255)                                                                                           gCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACg                           >  2:708686/1‑139 (MQ=255)                                                                                                aGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGt                      <  1:708686/139‑1 (MQ=255)                                                                                                aGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGt                      <  1:821293/139‑1 (MQ=255)                                                                                                aGCAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGt                      <  1:848905/139‑1 (MQ=255)                                                                                                  cAAGAAGCACTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCa                    <  2:445820/139‑1 (MQ=255)                                                                                                           cTTAAAAAATTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTtggt           >  2:545770/1‑139 (MQ=255)                                                                                                               aaaaaTTCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGct       <  2:444394/139‑1 (MQ=255)                                                                                                                    ttCGTTACACCAGGGAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGc  <  1:115379/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                             ACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGC  >  NZ_CP009273/2018652‑2018905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑