| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,370,899 | A→T | V58D (GTC→GAC) | menH ← | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,370,899 | 0 | A | T | 100.0% | 90.5 / NA | 23 | V58D (GTC→GAC) | menH | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (14/9); total (14/9) | |||||||||||
CATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAC > NZ_CP009273/2370790‑2371034 | catcatCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCt < 2:149409/139‑1 (MQ=255) atcatcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTg > 1:610728/1‑139 (MQ=255) gCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 2:610728/139‑1 (MQ=255) gCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 1:795923/139‑1 (MQ=255) tACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAaccac < 1:265453/103‑1 (MQ=255) tACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAaccac > 2:265453/1‑103 (MQ=255) caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATc < 2:433188/114‑1 (MQ=255) caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATc > 1:433188/1‑114 (MQ=255) caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGc > 1:170167/1‑138 (MQ=255) gccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGc > 2:419225/1‑139 (MQ=255) aaGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATcgccg > 2:15956/1‑79 (MQ=255) aaGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATcgccg < 1:15956/79‑1 (MQ=255) aaGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGc > 1:249374/1‑139 (MQ=255) aaGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCAct > 2:537743/1‑139 (MQ=255) tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGGGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGCGATCAACATACAACCGTGAGTAGTCGGCAACCGCCTCGCCCACTTCTTGc > 2:830752/1‑137 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 2:228945/72‑1 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt > 1:228945/1‑72 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTACCATTCGTGGCAATCGCCgg > 1:681192/1‑138 (MQ=255) cGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCa > 2:609422/1‑138 (MQ=255) tGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTgg < 1:847000/52‑1 (MQ=255) tGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTgg > 2:847000/1‑52 (MQ=255) aTCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATc < 2:237035/102‑1 (MQ=255) aTCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATc > 1:237035/1‑102 (MQ=255) tcgtcgCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAc > 2:560367/1‑139 (MQ=255) | CATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAC > NZ_CP009273/2370790‑2371034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |