Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	96,834	A→C	Q235P (CAA→CCA)	murG →	undecaprenyldiphospho‑muramoylpentapeptide beta‑N‑acetylglucosaminyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	96,834	0	A	C	100.0%	100.1 / NA	29	Q235P (CAA→CCA)	murG	undecaprenyldiphospho‑muramoylpentapeptide beta‑N‑acetylglucosaminyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/14);  total (15/14)

TTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTT  >  NZ_CP009273/96700‑96963                                                                                                                                       |                                                                                                                                  ttCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCg                                                                                                                               <  2:779108/139‑1 (MQ=255)                           aGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtga                                                                                                     <  1:352705/139‑1 (MQ=255)                             aCAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtgat                                                                                                    >  1:322000/1‑138 (MQ=255)                             aCAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtgat                                                                                                    >  1:795955/1‑138 (MQ=255)                                      cAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATggcgg                                                                                          <  2:703075/139‑1 (MQ=255)                                       aGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATggcggc                                                                                         >  2:844038/1‑139 (MQ=255)                                            gctgcGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGgcggcggcg                                                                                     <  2:324148/138‑1 (MQ=255)                                             ctgcGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTa                                                                                   <  1:28953/139‑1 (MQ=255)                                              tgcGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTAt                                                                                  >  1:315978/1‑139 (MQ=255)                                                       gggTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCg                                                                         >  1:725000/1‑139 (MQ=255)                                                        ggTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCgg                                                                        >  2:144597/1‑139 (MQ=255)                                                          tGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATggcggcg                                                                                        <  1:571135/121‑1 (MQ=255)                                                          tGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATggcggcg                                                                                        >  2:571135/1‑121 (MQ=255)                                                                       aTCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGGGGGCGGATGTCGTCGTTTGcc                                                         >  1:679367/1‑139 (MQ=255)                                                                            gCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCt                                                      <  2:291315/137‑1 (MQ=255)                                                                                          aaGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGgt                                      <  1:144597/139‑1 (MQ=255)                                                                                                      aaTCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATcgc                          <  1:473633/139‑1 (MQ=255)                                                                                                           gTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCgg                     <  1:733353/139‑1 (MQ=255)                                                                                                            ttGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGc                    <  2:555551/139‑1 (MQ=255)                                                                                                                    gCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGGATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTAc            >  1:686417/1‑139 (MQ=255)                                                                                                                         tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGc                    >  1:367814/1‑126 (MQ=255)                                                                                                                         tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGc                    <  2:367814/126‑1 (MQ=255)                                                                                                                         tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATCCGTGGGCGGGTGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGCGAAATCGCCGCGGCCGGACTACCgggg       >  1:712291/1‑137 (MQ=255)                                                                                                                         tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATGGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCg       <  2:259013/139‑1 (MQ=255)                                                                                                                              aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg                                                  <  1:280330/91‑1 (MQ=255)                                                                                                                              aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg                                                  >  2:280330/1‑91 (MQ=255)                                                                                                                              aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCgttgtt  >  2:302662/1‑139 (MQ=255)                                                                                                                              aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCgttgtt  >  1:564772/1‑139 (MQ=255)                                                                                                                              aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGGATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCgttgtt  >  1:325970/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                  TTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTT  >  NZ_CP009273/96700‑96963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑