Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,441,796	A→C	W42G (TGG→GGG)	prmB ←	50S ribosomal protein L3 N(5)‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,441,796	0	A	C	100.0%	143.8 / NA	41	W42G (TGG→GGG)	prmB	50S ribosomal protein L3 N(5)‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/19);  total (22/19)

GATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTC  >  NZ_CP009273/2441677‑2441928                                                                                                                        |                                                                                                                                     gATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGa                                                                                                                   <  2:493947/139‑1 (MQ=255)  aTCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGAc                                                                                                                  <  2:445457/139‑1 (MQ=255)       gcgTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTAc                                                                                                             <  2:357495/139‑1 (MQ=255)       gcgTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTAc                                                                                                             <  2:775200/139‑1 (MQ=255)       gcgTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTAc                                                                                                             <  2:667746/139‑1 (MQ=255)                     ggTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcgc                                                                                               >  1:338622/1‑139 (MQ=255)                     ggTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGGTATCGGTACCGTGACCGTACCAGATATTTGCcgc                                                                                               >  2:58798/1‑139 (MQ=255)                       tgtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCgcgc                                                                                             >  1:211164/1‑139 (MQ=255)                       tgtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCgcgc                                                                                             >  1:681814/1‑139 (MQ=255)                       tgtTTTACGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCgcgc                                                                                             >  1:834771/1‑139 (MQ=255)                            ttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAg                                                                                        >  2:301608/1‑139 (MQ=255)                            ttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAg                                                                                        >  1:305248/1‑139 (MQ=255)                            ttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTAACGTGACCGTACCAGATATTTGCCGCGCTGCAg                                                                                        >  2:800470/1‑139 (MQ=255)                                 tGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCt                                                                                   >  2:227439/1‑139 (MQ=255)                                         aGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc                                                                            <  2:305248/138‑1 (MQ=255)                                         aGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc                                                                            <  2:505970/138‑1 (MQ=255)                                            cgcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAgc                                                                        <  1:396248/139‑1 (MQ=255)                                                 ggTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAAc                                                                   <  1:301608/139‑1 (MQ=255)                                                  gTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACa                                                                  >  2:271061/1‑139 (MQ=255)                                                   tGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACAt                                                                 >  1:82805/1‑139 (MQ=255)                                                             ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCg                                                                                      <  1:736005/108‑1 (MQ=255)                                                             ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCg                                                                                      >  2:736005/1‑108 (MQ=255)                                                             ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATg                                                       >  1:766067/1‑139 (MQ=255)                                                             ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATg                                                       >  1:200838/1‑139 (MQ=255)                                                             ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGGTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATg                                                       >  1:130771/1‑139 (MQ=255)                                                               ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGaa                                                         <  1:227439/135‑1 (MQ=255)                                                               ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATg                                                       <  1:746797/137‑1 (MQ=255)                                                                cGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGtt                                                    >  1:644357/1‑139 (MQ=255)                                                                   aaTATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGc                                                 <  2:338622/139‑1 (MQ=255)                                                                       tCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGc                                              <  1:271061/138‑1 (MQ=255)                                                                        ccAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc                                                                            >  1:133299/1‑107 (MQ=255)                                                                        ccAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc                                                                            <  2:133299/107‑1 (MQ=255)                                                                                           aGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAAc                          >  1:296370/1‑138 (MQ=255)                                                                                            ggCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACg                         >  1:840912/1‑138 (MQ=255)                                                                                                     aGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTAt               <  2:257307/139‑1 (MQ=255)                                                                                                       cTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATcc             <  2:840912/139‑1 (MQ=255)                                                                                                            cGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGc                                              >  2:19680/1‑101 (MQ=255)                                                                                                            cGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGc                                              <  1:19680/101‑1 (MQ=255)                                                                                                             ggCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTAt       <  1:32171/139‑1 (MQ=255)                                                                                                               cTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATtc     >  1:765174/1‑139 (MQ=255)                                                                                                                  cATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATtcctc  >  2:292902/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                     GATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTC  >  NZ_CP009273/2441677‑2441928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑