| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,451,709 | G→T | S310R (AGC→AGA) | fadJ ← | fatty acid oxidation complex subunit alpha FadJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,451,709 | 0 | G | T | 91.4% | 111.3 / 3.8 | 35 | S310R (AGC→AGA) | fadJ | fatty acid oxidation complex subunit alpha FadJ |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base T (20/12); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
CAGCTGATCCCAACTGTACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCAT > NZ_CP009273/2451571‑2451842 | cAGCTGATCCCAACTGTACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACg < 2:349875/139‑1 (MQ=255) cccAACTGTACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTaa > 2:252990/1‑139 (MQ=255) tACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAg > 2:697550/1‑139 (MQ=255) tACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAg > 2:396814/1‑139 (MQ=255) tACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAg > 2:509945/1‑139 (MQ=255) tACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAg > 2:410977/1‑139 (MQ=255) cAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAggcggc > 2:699347/1‑139 (MQ=255) aGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGgcggcg < 2:172626/139‑1 (MQ=255) gATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACt > 1:783142/1‑139 (MQ=255) tGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCgg < 1:509945/138‑1 (MQ=255) tGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtctt > 2:303540/1‑139 (MQ=255) gCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAgg > 1:248891/1‑115 (MQ=255) gCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAgg < 2:248891/115‑1 (MQ=255) gCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtcttt > 2:65720/1‑139 (MQ=255) ggTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtctttctt < 1:699347/139‑1 (MQ=255) tttAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtct < 2:149738/124‑1 (MQ=255) tttAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtct > 1:149738/1‑124 (MQ=255) tAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTAc < 1:268893/138‑1 (MQ=255) cTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCa > 2:767632/1‑138 (MQ=255) aCCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaa > 2:822234/1‑138 (MQ=255) aCCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGc > 1:537597/1‑134 (MQ=255) aCCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGc < 2:537597/134‑1 (MQ=255) cgccgcCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAg < 2:783142/139‑1 (MQ=255) ccgccCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaaa < 2:237279/97‑1 (MQ=255) ccgccCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaaa > 1:237279/1‑97 (MQ=255) ccgccCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGtt > 2:592971/1‑139 (MQ=255) cgccCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTc > 2:817486/1‑139 (MQ=255) aTCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGTAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCaa < 1:287658/139‑1 (MQ=255) ccTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCtt > 1:503251/1‑139 (MQ=255) aaaTCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCTCGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCg < 1:252990/137‑1 (MQ=255) ccccACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCa > 2:361001/1‑139 (MQ=255) ccACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaaa < 2:443923/69‑1 (MQ=255) ccACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaaa > 1:443923/1‑69 (MQ=255) ccACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCAt > 1:563716/1‑138 (MQ=255) cACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATtgtg < 2:412111/95‑1 (MQ=255) cACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATtgtg > 1:412111/1‑95 (MQ=255) | CAGCTGATCCCAACTGTACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCAT > NZ_CP009273/2451571‑2451842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |