| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,482,405 | A→G | D81D (GAT→GAC) | yfdE ← | CoA:oxalate CoA‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,482,405 | 0 | A | G | 76.0% | 43.1 / 13.3 | 25 | D81D (GAT→GAC) | yfdE | CoA:oxalate CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (6/13); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-02 | |||||||||||
AGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTT > NZ_CP009273/2482270‑2482529 | aGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCa < 1:831635/139‑1 (MQ=255) cgGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAg > 2:422248/1‑138 (MQ=255) gggTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGaa < 1:756918/139‑1 (MQ=255) aTTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACcaca < 2:286914/138‑1 (MQ=255) aTTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACcaca < 1:902/138‑1 (MQ=255) aTTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACcaca < 1:607020/138‑1 (MQ=255) gCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGcc < 2:659194/139‑1 (MQ=255) tttCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCAt > 2:750870/1‑138 (MQ=255) aaaCCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATaaaa < 2:420903/139‑1 (MQ=255) cccAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGt < 1:422248/139‑1 (MQ=255) ggCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGcc < 1:303378/100‑1 (MQ=255) ggCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGcc > 2:303378/1‑100 (MQ=255) gAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATa < 1:681959/139‑1 (MQ=255) gAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATa < 2:806612/139‑1 (MQ=255) aaaaTTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAg > 2:433439/1‑139 (MQ=255) tACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCgg > 1:589521/1‑139 (MQ=255) cATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGt < 1:83431/139‑1 (MQ=255) aGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCa > 1:660703/1‑43 (MQ=255) aGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCa < 2:660703/43‑1 (MQ=255) gCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTatcat < 1:433439/139‑1 (MQ=255) tGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGtc < 1:822781/103‑1 (MQ=255) tGTTTGAGCATATTTATAAATATACTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGtc > 2:822781/1‑103 (MQ=255) tttATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATg < 1:81907/62‑1 (MQ=255) tttATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATg > 2:81907/1‑62 (MQ=255) ataCTTTTATCGTGGTCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACtt > 1:468917/1‑139 (MQ=255) | AGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTT > NZ_CP009273/2482270‑2482529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |