Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,484,113	A→G	S439S (TCT→TCC)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,484,113	0	A	G	100.0%	182.4 / NA	51	S439S (TCT→TCC)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/32);  total (19/32)

CACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCATTATCCAGGGTATTTGCAC  >  NZ_CP009273/2483983‑2484246                                                                                                                                   |                                                                                                                                      cacCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAg                                                                                                                                >  2:668364/1‑138 (MQ=255) cacCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAg                                                                                                                                >  2:47053/1‑138 (MQ=255)    cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                                            <  1:615159/139‑1 (MQ=255)    cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                                            <  1:567787/139‑1 (MQ=255)    cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                                            <  1:181404/139‑1 (MQ=255)    cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                                            <  1:47053/139‑1 (MQ=255)    cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                                            <  1:43241/139‑1 (MQ=255)    cGTCTCCTCTGGAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGCACCAGAAGt                                                                                                                            >  2:315549/1‑139 (MQ=255)      tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCa                                                                                                                          >  2:335675/1‑139 (MQ=255)      tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCa                                                                                                                          >  2:299551/1‑139 (MQ=255)                 gATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGTCAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                                               <  1:747788/139‑1 (MQ=255)                 gATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                                               <  1:185198/139‑1 (MQ=255)                  aTGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGa                                                                                                              <  1:668364/139‑1 (MQ=255)                    gccgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATg                                                                                                            >  1:307139/1‑139 (MQ=255)                    gccgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATg                                                                                                            >  2:728618/1‑139 (MQ=255)                      cgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                                          <  1:478419/139‑1 (MQ=255)                      cgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                                          <  2:694729/139‑1 (MQ=255)                       gccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                                         <  1:54558/139‑1 (MQ=255)                         cATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                                         >  2:51804/1‑137 (MQ=255)                                 aaaaTAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATAc                                                                                               <  1:299551/139‑1 (MQ=255)                                             gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                                               <  1:424324/111‑1 (MQ=255)                                             gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                                               >  2:424324/1‑111 (MQ=255)                                             gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                                   <  1:335675/139‑1 (MQ=255)                                             gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                                   >  1:95475/1‑139 (MQ=255)                                                aCCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                                          <  2:231602/113‑1 (MQ=255)                                                aCCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                                          >  1:231602/1‑113 (MQ=255)                                                  cGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                                         <  2:493058/112‑1 (MQ=255)                                                  cGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                                         >  1:493058/1‑112 (MQ=255)                                                        gTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTa                                                                        >  1:764783/1‑139 (MQ=255)                                                                gACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATc                                                                <  2:95475/139‑1 (MQ=255)                                                                  cAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                                            <  2:444334/77‑1 (MQ=255)                                                                  cAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                                            >  1:444334/1‑77 (MQ=255)                                                                            cAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     <  2:611277/138‑1 (MQ=255)                                                                            cAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     <  2:307139/138‑1 (MQ=255)                                                                            cAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     <  2:611261/138‑1 (MQ=255)                                                                            cAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     <  1:51804/138‑1 (MQ=255)                                                                             aaTTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGacgacgacg                                                    <  1:715327/138‑1 (MQ=255)                                                                                     tCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTtata                                           <  1:769052/139‑1 (MQ=255)                                                                                        cACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACat                                        <  1:728618/139‑1 (MQ=255)                                                                                           tGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACatat                                      >  2:501788/1‑138 (MQ=255)                                                                                                           aTCACCGTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCAAAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCa                     <  2:235179/139‑1 (MQ=255)                                                                                                                 ttCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                                                                             >  1:509479/1‑61 (MQ=255)                                                                                                                 ttCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                                                                             <  2:509479/61‑1 (MQ=255)                                                                                                                      tGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                                          >  1:220129/1‑43 (MQ=255)                                                                                                                      tGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                                          <  2:220129/43‑1 (MQ=255)                                                                                                                      tGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     <  2:217962/96‑1 (MQ=255)                                                                                                                      tGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     >  1:217962/1‑96 (MQ=255)                                                                                                                         cGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     >  2:195879/1‑93 (MQ=255)                                                                                                                         cGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                                     <  1:195879/93‑1 (MQ=255)                                                                                                                          gACAACCGGGGAGCCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCATTATCCAGGGTAttt      <  1:525981/139‑1 (MQ=255)                                                                                                                              aCCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCATTATCCAGGGTATTTGCAc  <  2:78218/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      CACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCATTATCCAGGGTATTTGCAC  >  NZ_CP009273/2483983‑2484246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑