Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,595,868	T→C	L231S (TTG→TCG)	hyfB →	hydrogenase 4 subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,595,868	0	T	C	100.0%	112.9 / NA	34	L231S (TTG→TCG)	hyfB	hydrogenase 4 subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/23);  total (11/23)

GCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTTGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGG  >  NZ_CP009273/2595734‑2596001                                                                                                                                       |                                                                                                                                      gcgcGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCcg                                                                                                                                   <  1:218447/139‑1 (MQ=255)     gAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCcgcgcg                                                                                                                               <  1:730597/139‑1 (MQ=255)         gCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCa                                                                                                                           <  2:522703/139‑1 (MQ=255)         gCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCa                                                                                                                           <  2:54019/139‑1 (MQ=255)                           ccAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCa                                                                                                         <  1:759532/139‑1 (MQ=255)                           ccAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCa                                                                                                         <  1:677496/139‑1 (MQ=255)                                 tCCGCACGTTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCtt                                                                                                    <  2:769186/138‑1 (MQ=255)                                 tCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCtt                                                                                                    <  1:3508/138‑1 (MQ=255)                                 tCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCtt                                                                                                    <  2:491137/138‑1 (MQ=255)                                 tCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCtt                                                                                                    <  2:476693/138‑1 (MQ=255)                                                    tcCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGGTGCACAGCTGGCCGCCGCGGGCTCACCCTGCCGCACCAACGCACCCTTCGGCGGTGATGTCTGGCGt                                                                                 >  1:827280/1‑138 (MQ=255)                                                            tGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCa                                                                         >  1:686052/1‑138 (MQ=255)                                                                             ctgctgGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGc                                                        <  1:392029/138‑1 (MQ=255)                                                                               gctgGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGGAATGGTCAAAATAGGAATTTTCGGCATc                                                     >  1:590784/1‑139 (MQ=255)                                                                                  ggCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTa                                                                                >  2:795601/1‑109 (MQ=255)                                                                                  ggCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTa                                                                                <  1:795601/109‑1 (MQ=255)                                                                                   gCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGa                                                 <  2:638042/139‑1 (MQ=255)                                                                                     cTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCTGTTGCACAGCGGGTCGCAGCGCGCTCAACCTGCCGCACCATAGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGaaa                                               <  2:24773/139‑1 (MQ=255)                                                                                             gTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGAt                                       <  2:686052/139‑1 (MQ=255)                                                                                               tttGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCa                                                                                                         <  2:386676/71‑1 (MQ=255)                                                                                               tttGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCAACCTGCCGCACCATCGCa                                                                                                         >  1:386676/1‑71 (MQ=255)                                                                                                  ggCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATc                                  >  1:53573/1‑139 (MQ=255)                                                                                                                    atgCCGTTGTCCAGCTGGTCGCCGCGCGCTCCCCCTGCCGCACCATCGCCCGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGtt                <  2:156643/139‑1 (MQ=255)                                                                                                                    atgCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGtt                <  1:236675/139‑1 (MQ=255)                                                                                                                     tgCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGttt               >  1:403394/1‑139 (MQ=255)                                                                                                                       ccGTTGCCCAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGc             <  2:827261/139‑1 (MQ=255)                                                                                                                          ttGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCctc          <  2:403394/139‑1 (MQ=255)                                                                                                                          ttGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCctc          >  2:543355/1‑139 (MQ=255)                                                                                                                          ttGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCctc          >  1:574166/1‑139 (MQ=255)                                                                                                                              acaGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCtgtg      <  1:172099/139‑1 (MQ=255)                                                                                                                                 gCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTgtggtg   <  2:574166/139‑1 (MQ=255)                                                                                                                                  cTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGtt                <  2:202135/125‑1 (MQ=255)                                                                                                                                  cTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGtt                >  1:202135/1‑125 (MQ=255)                                                                                                                                  cTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGtggtgg  >  1:810942/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      GCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTTGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGG  >  NZ_CP009273/2595734‑2596001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑