Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	100,067	A→G	G142G (GGA→GGG)	ftsQ →	cell division protein FtsQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	100,067	0	A	G	100.0%	177.2 / NA	48	G142G (GGA→GGG)	ftsQ	cell division protein FtsQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

CGCAAATAGAACAACGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATT  >  NZ_CP009273/99931‑100198                                                                                                                                         |                                                                                                                                    cGCAAATAGAACAACGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGgaa                                                                                                                                   <  2:631091/139‑1 (MQ=255) cGCAAATAGAACAACGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGgaa                                                                                                                                   <  2:56019/139‑1 (MQ=255)               cGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTgc                                                                                                                     >  1:99451/1‑139 (MQ=255)                  cTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTgccgc                                                                                                                  <  2:306767/139‑1 (MQ=255)                    gCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTgccgc                                                                                                                  >  1:364748/1‑137 (MQ=255)                                    gTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCagcaag                                                                                                 >  1:787451/1‑138 (MQ=255)                                    gTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCagcaag                                                                                                 >  2:478751/1‑138 (MQ=255)                                        gCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            <  2:99451/139‑1 (MQ=255)                                        gCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            <  1:711947/139‑1 (MQ=255)                                        gCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            <  2:453666/139‑1 (MQ=255)                                        gCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            <  1:613837/139‑1 (MQ=255)                                             aGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTc                                                                                       <  1:752142/139‑1 (MQ=255)                                                  gCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATg                                                                                  <  1:728467/139‑1 (MQ=255)                                                        gCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTa                                                                             <  1:679016/138‑1 (MQ=255)                                                        gCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTa                                                                             <  2:384460/138‑1 (MQ=255)                                                           tgatgaATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc                                                                         >  1:667740/1‑139 (MQ=255)                                                                       gATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTc                                                                                       <  2:438642/113‑1 (MQ=255)                                                                       gATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTc                                                                                       >  1:438642/1‑113 (MQ=255)                                                                          tCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAgcgc                                                           >  1:620736/1‑138 (MQ=255)                                                                            aTCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCaa                                                        >  1:344680/1‑139 (MQ=255)                                                                                 gTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTa                                                                             >  2:538664/1‑113 (MQ=255)                                                                                 gTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTa                                                                             <  1:538664/113‑1 (MQ=255)                                                                                 gTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGaa                                                    <  2:344680/138‑1 (MQ=255)                                                                                 gTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAg                                                   <  2:787451/139‑1 (MQ=255)                                                                                      atatGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTg                                              <  2:266826/139‑1 (MQ=255)                                                                                           tgacGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAggg                                         >  2:485645/4‑139 (MQ=255)                                                                                           tgCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGaa                                                    >  1:646158/1‑128 (MQ=255)                                                                                           tgCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGaa                                                    <  2:646158/128‑1 (MQ=255)                                                                                           tgCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAggg                                         >  2:18065/1‑139 (MQ=255)                                                                                                aTTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATc                                    >  1:113002/1‑139 (MQ=255)                                                                                                aTTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATc                                    >  2:845471/1‑139 (MQ=255)                                                                                                 ttGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATcg                                   <  2:507642/139‑1 (MQ=255)                                                                                                    cgcgGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATcgcg                                 >  2:847995/1‑138 (MQ=255)                                                                                                              tGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAg                      <  2:393792/139‑1 (MQ=255)                                                                                                                   aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTc                                                                                       <  2:94234/69‑1 (MQ=255)                                                                                                                   aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTc                                                                                       >  1:94234/1‑69 (MQ=255)                                                                                                                   aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATg                                                      >  1:220727/1‑102 (MQ=255)                                                                                                                   aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATg                                                      <  2:220727/102‑1 (MQ=255)                                                                                                                   aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCt                 >  2:642648/1‑139 (MQ=255)                                                                                                                       tatGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCa             <  2:667740/139‑1 (MQ=255)                                                                                                                            tAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa        <  2:620736/139‑1 (MQ=255)                                                                                                                            tAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa        >  1:61137/1‑139 (MQ=255)                                                                                                                               aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            >  1:509659/1‑52 (MQ=255)                                                                                                                               aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            <  2:509659/52‑1 (MQ=255)                                                                                                                               aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATcg                                   >  2:632352/1‑109 (MQ=255)                                                                                                                               aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATcg                                   <  1:632352/109‑1 (MQ=255)                                                                                                                               aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa        >  1:45952/1‑136 (MQ=255)                                                                                                                                 gcgGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGAt   <  1:598222/139‑1 (MQ=255)                                                                                                                                 gcgGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGAt   <  2:364748/139‑1 (MQ=255)                                                                                                                                  cgGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGAtt  <  2:113002/139‑1 (MQ=255)                                                                                                                                      agggaATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            <  1:595930/45‑1 (MQ=255)                                                                                                                                      agggaATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                            >  2:595930/1‑45 (MQ=255)                                                                                                                                         |                                                                                                                                    CGCAAATAGAACAACGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATT  >  NZ_CP009273/99931‑100198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑