| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,636,307 | A→G | intergenic (‑104/+181) | rodZ ← / ← rlmN | cytoskeleton protein RodZ/bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,636,307 | 0 | A | G | 80.6% | 81.7 / 11.8 | 36 | intergenic (‑104/+181) | rodZ/rlmN | cytoskeleton protein RodZ/bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base G (12/17); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCAT > NZ_CP009273/2636170‑2636437 | cTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGAc < 2:662740/139‑1 (MQ=255) ttCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGcc > 2:769797/1‑139 (MQ=255) ttttGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCt < 2:606352/139‑1 (MQ=255) tGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCtgtg > 2:731601/1‑139 (MQ=255) gTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCtgtgtg > 2:413574/1‑139 (MQ=255) tCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGc < 2:12784/139‑1 (MQ=255) cGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACt > 2:803104/1‑138 (MQ=255) cGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACt < 1:731601/138‑1 (MQ=255) cTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACt > 2:160928/1‑137 (MQ=255) cAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAgg < 2:671904/137‑1 (MQ=255) cAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGc < 1:782717/138‑1 (MQ=255) cGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTa < 1:732760/139‑1 (MQ=255) tttAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACt > 1:61584/1‑139 (MQ=255) tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa < 1:198991/139‑1 (MQ=255) tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa < 1:628300/139‑1 (MQ=255) tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa < 2:483486/139‑1 (MQ=255) tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa < 2:40784/139‑1 (MQ=255) tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa < 2:61584/139‑1 (MQ=255) aaTTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCcaca > 1:292946/1‑109 (MQ=255) aaTTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCcaca < 2:292946/109‑1 (MQ=255) aaTTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGt > 1:166744/1‑139 (MQ=255) gaTGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATa < 1:829796/139‑1 (MQ=255) gaTGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATa < 1:850228/139‑1 (MQ=255) ccGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACa > 2:358548/1‑90 (MQ=255) ccGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACa < 1:358548/90‑1 (MQ=255) tcatGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAg > 1:30977/1‑139 (MQ=255) cccACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGc < 2:166744/139‑1 (MQ=255) cgAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTaa > 1:66515/1‑138 (MQ=255) cgAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTaa > 1:781005/1‑138 (MQ=255) cgAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTaa > 1:273897/1‑138 (MQ=255) cgAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTaa > 1:727965/1‑138 (MQ=255) ttaagttaaCCG‑TTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGAc < 2:781005/139‑1 (MQ=255) tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACt > 2:702413/1‑52 (MQ=255) tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACt < 1:702413/52‑1 (MQ=255) tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACttcat > 2:727528/1‑137 (MQ=255) tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACttcat < 1:727528/137‑1 (MQ=255) | CTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCG‑TTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCAT > NZ_CP009273/2636170‑2636437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |