Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,705,165	G→A	A463T (GCC→ACC)	nadB →	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,705,165	0	G	A	100.0%	156.5 / NA	42	A463T (GCC→ACC)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/19);  total (23/19)

TTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAATGA  >  NZ_CP009273/2705033‑2705294                                                                                                                                     |                                                                                                                                  ttACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTgc                                                                                                                             >  1:256229/1‑139 (MQ=255)  tACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTgcg                                                                                                                            >  1:627483/1‑139 (MQ=255)       ccgTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGa                                                                                                                       <  2:41183/139‑1 (MQ=255)       ccgTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGa                                                                                                                       >  1:545223/1‑139 (MQ=255)            ggATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAAc                                                                                                                   <  1:235148/138‑1 (MQ=255)                 aaaGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCt                                                                                                             >  2:685998/1‑139 (MQ=255)                                 cccTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGAc                                                                                             <  1:331647/139‑1 (MQ=255)                                  ccTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACg                                                                                            <  2:313319/139‑1 (MQ=255)                                      aCGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAata                                                                                        <  2:648798/139‑1 (MQ=255)                                           cGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACg                                                                                   >  1:188021/1‑139 (MQ=255)                                                    aTTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACACTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTcc                                                                          <  1:320668/139‑1 (MQ=255)                                                        aGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGt                                                                      >  2:115696/1‑139 (MQ=255)                                                        aGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGt                                                                      >  2:334644/1‑139 (MQ=255)                                                          cATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTgcg                                                                                                                            >  2:324999/1‑83 (MQ=255)                                                          cATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTgcg                                                                                                                            <  1:324999/83‑1 (MQ=255)                                                          cATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtct                                                                    >  1:539201/1‑139 (MQ=255)                                                            tAACTGGCACGAGCTACGTCTGTTTATGTGGGCTTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtctc                                                                   >  1:754602/1‑138 (MQ=255)                                                               cTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTcc                                                                          <  1:674490/128‑1 (MQ=255)                                                               cTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTcc                                                                          >  2:674490/1‑128 (MQ=255)                                                               cTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCcaa                                                                >  2:524951/1‑138 (MQ=255)                                                                    aCGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAAttt                                                          >  1:829549/1‑139 (MQ=255)                                                                            cGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAg                                                   <  2:539201/138‑1 (MQ=255)                                                                                  tttATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGt                                             >  2:608826/1‑138 (MQ=255)                                                                                  tttATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGt                                             >  1:236861/1‑138 (MQ=255)                                                                                     aTGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAAt                                          <  1:71581/138‑1 (MQ=255)                                                                                     aTGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAAt                                          <  1:608826/138‑1 (MQ=255)                                                                                          ggATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGC‑‑‑TAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAg                                 >  1:596514/1‑139 (MQ=255)                                                                                           gATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTAc                                   <  2:216431/139‑1 (MQ=255)                                                                                           gATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTAc                                   <  1:334644/139‑1 (MQ=255)                                                                                                    ggCATTGTGCGCACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGcc                           >  2:112106/1‑138 (MQ=255)                                                                                                             cgcACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTg                 >  2:2943/1‑139 (MQ=255)                                                                                                              gcACAACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGt                <  2:787039/139‑1 (MQ=255)                                                                                                               cacaACGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGtt               <  2:545223/139‑1 (MQ=255)                                                                                                                    cGAAGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtg          >  1:457601/1‑139 (MQ=255)                                                                                                                       aGCGCCTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgtg        >  1:93508/1‑138 (MQ=255)                                                                                                                        gcgtCTGGAACGTACCCTGCGGCGGATAACCATGCTCCAACAAGCACTAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGACTTGATTGTTCGCTGTGCa      <  2:191980/139‑1 (MQ=255)                                                                                                                            cTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCg                                              <  2:358082/95‑1 (MQ=255)                                                                                                                            cTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCg                                              >  1:358082/1‑95 (MQ=255)                                                                                                                            cTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATc                                         >  1:693882/1‑100 (MQ=255)                                                                                                                            cTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATc                                         <  2:693882/100‑1 (MQ=255)                                                                                                                            cTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAatga  >  2:625445/1‑139 (MQ=255)                                                                                                                            cTGGAACGCACCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAatga  <  1:685998/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  TTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAATGA  >  NZ_CP009273/2705033‑2705294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑