Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,718,722	A→G	L128L (CTT→CTC)	kgtP ←	alpha‑ketoglutarate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,718,722	0	A	G	100.0%	100.2 / NA	28	L128L (CTT→CTC)	kgtP	alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/17);  total (11/17)

GTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCC  >  NZ_CP009273/2718589‑2718856                                                                                                                                      |                                                                                                                                       gTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCaa                                                                                                                                   >  2:852750/1‑139 (MQ=255) gTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCaa                                                                                                                                   >  1:823411/1‑139 (MQ=255)    cgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCaataa                                                                                                                                >  2:532979/1‑139 (MQ=255)                tCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCa                                                                                                                    <  2:823411/139‑1 (MQ=255)                  acaTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACg                                                                                                                  <  1:501812/139‑1 (MQ=255)                   caTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACg                                                                                                                  >  1:489179/1‑138 (MQ=255)                                  cGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACgg                                                                                                                 >  1:430340/1‑123 (MQ=255)                                  cGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACgg                                                                                                                 <  2:430340/124‑2 (MQ=255)                                              tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTg                                                                                             >  2:781283/1‑132 (MQ=255)                                              tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTg                                                                                             <  1:781283/132‑1 (MQ=255)                                              tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGaggc                                                                                       <  2:548706/138‑1 (MQ=255)                                                              aaCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGAt                                                                      <  2:764846/139‑1 (MQ=255)                                                                    aCTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa                                                                <  2:531691/139‑1 (MQ=255)                                                                    aCTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa                                                                 <  1:297099/138‑1 (MQ=255)                                                                     cTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAac                                                               <  1:532979/139‑1 (MQ=255)                                                                        atatAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAAcaca                                                            <  1:656148/139‑1 (MQ=255)                                                                              gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac                                                      <  2:767606/139‑1 (MQ=255)                                                                              gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac                                                      <  2:71400/139‑1 (MQ=255)                                                                              gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac                                                      <  1:291125/139‑1 (MQ=255)                                                                                  cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa                                                                                                    <  2:382590/89‑1 (MQ=255)                                                                                  cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa                                                                                                    >  1:382590/1‑89 (MQ=255)                                                                                  cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac                                                  >  1:383372/1‑139 (MQ=255)                                                                                  cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac                                                  >  1:136803/1‑139 (MQ=255)                                                                                                  tcCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAt                                  >  2:664443/1‑139 (MQ=255)                                                                                                            gATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGAc                        <  2:383372/139‑1 (MQ=255)                                                                                                                     tGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTAt               >  1:10246/1‑139 (MQ=255)                                                                                                                            aaCGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTa         <  2:489179/138‑1 (MQ=255)                                                                                                                                  cgagGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGcc  <  1:664443/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCC  >  NZ_CP009273/2718589‑2718856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑