Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,718,745	T→G	T121P (ACG→CCG)	kgtP ←	alpha‑ketoglutarate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,718,745	0	T	G	100.0%	79.1 / NA	22	T121P (ACG→CCG)	kgtP	alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/15);  total (7/15)

TGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCC  >  NZ_CP009273/2718634‑2718856                                                                                                                |                                                                                                                tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTg                                                                                             >  2:781283/1‑132 (MQ=255) tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTg                                                                                             <  1:781283/132‑1 (MQ=255) tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGaggc                                                                                       <  2:548706/138‑1 (MQ=255)                 aaCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGAt                                                                      <  2:764846/139‑1 (MQ=255)                       aCTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa                                                                <  2:531691/139‑1 (MQ=255)                       aCTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa                                                                 <  1:297099/138‑1 (MQ=255)                        cTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAac                                                               <  1:532979/139‑1 (MQ=255)                           atatAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAAcaca                                                            <  1:656148/139‑1 (MQ=255)                                 gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac                                                      <  1:291125/139‑1 (MQ=255)                                 gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac                                                      <  2:767606/139‑1 (MQ=255)                                 gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac                                                      <  2:71400/139‑1 (MQ=255)                                     cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa                                                                                                    <  2:382590/89‑1 (MQ=255)                                     cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa                                                                                                    >  1:382590/1‑89 (MQ=255)                                     cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac                                                  >  1:383372/1‑139 (MQ=255)                                     cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac                                                  >  1:136803/1‑139 (MQ=255)                                                     tcCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAt                                  >  2:664443/1‑139 (MQ=255)                                                               gATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGAc                        <  2:383372/139‑1 (MQ=255)                                                                        tGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTAt               >  1:10246/1‑139 (MQ=255)                                                                               aaCGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTa         <  2:489179/138‑1 (MQ=255)                                                                                     cgagGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGcc  <  1:664443/139‑1 (MQ=255)                                                                                                    ccGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatcat                                                        >  2:124603/1‑70 (MQ=255)                                                                                                    ccGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatcat                                                        <  1:124603/70‑1 (MQ=255)                                                                                                                |                                                                                                                TGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCC  >  NZ_CP009273/2718634‑2718856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑