Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,724,669	T→G	intergenic (‑153/+290)	BW25113_RS13535 ← / ← clpB	16S ribosomal RNA/ATP‑dependent chaperone ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,724,669	0	T	G	100.0%	15.2 / NA	6	intergenic (‑153/+290)	BW25113_RS13535/clpB	16S ribosomal RNA/ATP‑dependent chaperone ClpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/4);  total (2/4)

ACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTC  >  NZ_CP009273/2724600‑2724804                                                                      |                                                                                                                                        aCGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCt                                                                    <  1:613246/139‑1 (MQ=21)    ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                                 <  2:146955/139‑1 (MQ=255)                           cccACACAGATTGTCTGATAAGTTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga                                           <  1:611297/138‑1 (MQ=255)                                    aTTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGa                                 >  2:293033/1‑139 (MQ=255)                                    aTTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGa                                 >  2:469446/1‑139 (MQ=255)                                            aTAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                         <  1:469446/139‑1 (MQ=255)                                                                   gctgcgctcTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTc  <  1:669240/139‑1 (MQ=255)                                                                      |                                                                                                                                        ACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTC  >  NZ_CP009273/2724600‑2724804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑