Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,724,674	T→C	intergenic (‑158/+285)	BW25113_RS13535 ← / ← clpB	16S ribosomal RNA/ATP‑dependent chaperone ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,724,674	0	T	C	100.0%	15.8 / NA	6	intergenic (‑158/+285)	BW25113_RS13535/clpB	16S ribosomal RNA/ATP‑dependent chaperone ClpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/4);  total (2/4)

ACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGT  >  NZ_CP009273/2724600‑2724807                                                                           |                                                                                                                                      aCGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCt                                                                       <  1:613246/139‑1 (MQ=21)    ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                                                                    <  2:146955/139‑1 (MQ=255)                           cccACACAGATTGTCTGATAAGTTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga                                              <  1:611297/138‑1 (MQ=255)                                    aTTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGa                                    >  2:293033/1‑139 (MQ=255)                                    aTTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGa                                    >  2:469446/1‑139 (MQ=255)                                            aTAAATTGTTAAAGAGCAGTGCCGCTGCGCTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCcggcgg                            <  1:469446/139‑1 (MQ=255)                                                                   gctgcgctcTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTc     <  1:669240/139‑1 (MQ=255)                                                                      gcgctcTTTCTCAGCGGCGCAGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAgt  >  2:28438/2‑139 (MQ=255)                                                                           |                                                                                                                                      ACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGT  >  NZ_CP009273/2724600‑2724807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑