Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,923,200 A→G D89G (GAT→GGT)  sdaB → L‑serine ammonia‑lyase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,923,2000AG100.0% 169.4 / NA 44D89G (GAT→GGT) sdaBL‑serine ammonia‑lyase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

CGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTA  >  NZ_CP009273/2923066‑2923322
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cgTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATAACGTGGATATCGATTCCATCCCCAGTTTTATTCAGGgtgt                                                                                                                         >  2:456602/1‑138 (MQ=255)
 gtggtgGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGa                                                                                                                       <  1:456602/139‑1 (MQ=255)
       gtTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGa                                                                                                                       >  1:714618/1‑133 (MQ=255)
       gtTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGa                                                                                                                       <  2:714618/133‑1 (MQ=255)
               gtACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGc                                                                                                         >  1:677479/1‑139 (MQ=255)
               gtACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGc                                                                                                         >  1:804760/1‑139 (MQ=255)
                              tctGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCaa                                                                                            <  2:169384/137‑1 (MQ=255)
                              tctGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCATGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCa                                                                                             <  2:503624/136‑1 (MQ=255)
                               ctGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATAGATTCCATCCCCAGCTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACg                                                                                         >  2:253045/1‑139 (MQ=255)
                                  aCCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGt                                                                                       <  2:54223/138‑1 (MQ=255)
                                   ccGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGt                                                                                       >  2:290917/1‑137 (MQ=255)
                                             ccaccaCACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTg                                                                           <  1:542613/139‑1 (MQ=255)
                                                 cacaCTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGt                                                                       >  2:207844/1‑139 (MQ=255)
                                                 cacaCTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGt                                                                       >  1:572014/1‑139 (MQ=255)
                                                  acacTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGtt                                                                      <  2:457093/139‑1 (MQ=255)
                                                        atatCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGt                                                                <  1:231827/139‑1 (MQ=255)
                                                           tCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATgg                                                                                                             >  2:530943/1‑91 (MQ=255)
                                                           tCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATgg                                                                                                             <  1:530943/91‑1 (MQ=255)
                                                             gCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGt                                                                                       <  2:489039/111‑1 (MQ=255)
                                                             gCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGt                                                                                       >  1:489039/1‑111 (MQ=255)
                                                             gCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGAt                                                            <  1:290917/138‑1 (MQ=255)
                                                             gCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGAt                                                            <  1:207844/138‑1 (MQ=255)
                                                                  tattatGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATg                                                                                                              <  1:778266/83‑1 (MQ=255)
                                                                  tattatGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATg                                                                                                              >  2:778266/1‑83 (MQ=255)
                                                                       tGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGaa                                                 >  1:571996/1‑139 (MQ=255)
                                                                              ggCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGt                                                                                       <  1:154385/94‑1 (MQ=255)
                                                                              ggCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGAt                                                            <  2:553937/121‑1 (MQ=255)
                                                                              ggCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGAt                                                            >  1:553937/1‑121 (MQ=255)
                                                                              ggCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATg                                                   >  1:331696/1‑130 (MQ=255)
                                                                              ggCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATg                                                   <  2:331696/130‑1 (MQ=255)
                                                                              ggCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCAc                                          >  1:343722/1‑139 (MQ=255)
                                                                              ggCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCAc                                          >  2:331537/1‑139 (MQ=255)
                                                                              ggCGGGGAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGt                                                                                       >  2:154385/1‑94 (MQ=255)
                                                                                                     tGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTg                                                                           >  1:559750/1‑83 (MQ=255)
                                                                                                     tGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTg                                                                           <  2:559750/83‑1 (MQ=255)
                                                                                                     tGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATg                                                   <  1:770451/107‑1 (MQ=255)
                                                                                                     tGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATg                                                   >  2:770451/1‑107 (MQ=255)
                                                                                                     tGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATg                    >  2:53205/1‑138 (MQ=255)
                                                                                                     tGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATg                    >  2:849154/1‑138 (MQ=255)
                                                                                                             gATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGt            <  2:571996/138‑1 (MQ=255)
                                                                                                             gATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGt            <  1:53205/138‑1 (MQ=255)
                                                                                                                  cATCCCCAGTTCTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATgcg       <  1:593031/138‑1 (MQ=255)
                                                                                                                    tCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCAt    >  2:726995/1‑139 (MQ=255)
                                                                                                                       ccAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTa  >  2:232464/1‑138 (MQ=255)
                                                                                                                       ccAGTTTTATTCAGGGTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTa  <  1:232464/138‑1 (MQ=255)
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CGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTA  >  NZ_CP009273/2923066‑2923322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑