| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 123,341 | T→A | L387Q (CTG→CAG) | aceF → | pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 123,341 | 0 | T | A | 96.4% | 101.2 / NA | 28 | L387Q (CTG→CAG) | aceF | pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (12/15); minor base C (1/0); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAAC > NZ_CP009273/123214‑123476 | gCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCCGCCGGGGCCg > 2:541470/1‑138 (MQ=255) tGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAg > 1:180790/1‑139 (MQ=255) tGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAg > 1:773957/1‑139 (MQ=255) tAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAgg > 1:292755/1‑119 (MQ=255) tAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAgg < 2:292755/119‑1 (MQ=255) cTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGaagaag < 2:754722/139‑1 (MQ=255) cgcgAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCga > 1:229317/1‑131 (MQ=255) cgcgAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCga < 2:229317/131‑1 (MQ=255) cgcgAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGGTTGGGGAAATCGAAGAAGTgg > 2:419155/1‑139 (MQ=255) cGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGt < 2:107967/74‑1 (MQ=255) cGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGa < 2:375793/80‑1 (MQ=255) cGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGaa < 1:531312/138‑1 (MQ=255) cGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCCGGCATGCAGCCGTGGCCGa > 1:375793/1‑80 (MQ=255) tCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCg < 2:180790/137‑1 (MQ=255) aaaCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAAcc < 1:205924/139‑1 (MQ=255) cAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATTCCTGGCATGCAGCCGTGGCCGAAgg > 2:372499/1‑60 (MQ=39) cAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATTCCTGGCATGCAGCCGTGGCCGAAgg < 1:372499/60‑1 (MQ=39) gcTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTGGAAATGGGCCGCATCCAGAAAATCTCTGGGGCGAACCTGAGCCGTAACTggg > 2:671205/1‑139 (MQ=255) ggCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAAt > 2:547696/1‑138 (MQ=255) ggCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATg < 1:547696/139‑1 (MQ=255) cGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATccc < 1:63249/139‑1 (MQ=255) gCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGc < 2:489760/135‑1 (MQ=255) gCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGc > 1:489760/1‑135 (MQ=255) ggtggtATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTa < 2:444180/139‑1 (MQ=255) tggtATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACt < 2:354715/139‑1 (MQ=255) tCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACt < 2:515590/138‑1 (MQ=255) ccTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTc > 2:784765/1‑138 (MQ=255) ggCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGAc > 1:696252/1‑138 (MQ=255) tgcagcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAAc < 2:3129/139‑1 (MQ=255) | GCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAAC > NZ_CP009273/123214‑123476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |