Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,050,507	A→G	intergenic (‑199/+30)	ibsC ← / ← serA	type I toxin‑antitoxin system toxin IbsC/phosphoglycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,050,507	0	A	G	100.0%	110.7 / NA	30	intergenic (‑199/+30)	ibsC/serA	type I toxin‑antitoxin system toxin IbsC/phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

GTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCA  >  NZ_CP009273/3050378‑3050634                                                                                                                                  |                                                                                                                                gTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGt                                                                                                                        >  2:383018/1‑139 (MQ=255)    aaTCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTg                                                                                                                      <  1:490709/138‑1 (MQ=255)      tacccATTAAATAAACACGACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGAAAAGTAGAATGGGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGAtt                                                                                                                   >  2:481342/3‑139 (MQ=255)                 taaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                        <  2:653296/139‑1 (MQ=255)                  aaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAggg                                                                                                       >  1:791999/1‑139 (MQ=255)                             aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                                                              >  2:7979/1‑137 (MQ=255)                                      aCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                                                                   >  1:327529/1‑139 (MQ=255)                                           gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                        <  2:453440/113‑1 (MQ=255)                                           gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                        >  1:453440/1‑113 (MQ=255)                                            atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGaa                                                                             <  2:212993/139‑1 (MQ=255)                                            atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGaa                                                                             >  1:212993/1‑139 (MQ=255)                                                         aGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                                                                <  2:767790/139‑1 (MQ=255)                                                           cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                                                              <  2:593564/139‑1 (MQ=255)                                                                        tAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                 >  1:709179/1‑139 (MQ=255)                                                                            aTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGtt                                                                                                                       >  2:741717/1‑65 (MQ=255)                                                                            aTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGtt                                                                                                                       <  1:741717/65‑1 (MQ=255)                                                                                       ggCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGc                                  <  2:327529/139‑1 (MQ=255)                                                                                        gCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCa                                 <  1:180106/139‑1 (MQ=255)                                                                                                 tgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcg                        >  2:73003/1‑139 (MQ=255)                                                                                                   gagaAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcgt                       <  1:770831/138‑1 (MQ=255)                                                                                                      aaGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                                                      >  2:251464/1‑104 (MQ=255)                                                                                                      aaGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                                                      <  1:251464/104‑1 (MQ=255)                                                                                                      aaGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGc                   >  1:500655/1‑139 (MQ=255)                                                                                                         ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                 >  1:40081/1‑138 (MQ=255)                                                                                                         ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                 >  1:374297/1‑138 (MQ=255)                                                                                                          gATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtca               <  2:591763/139‑1 (MQ=255)                                                                                                           aTAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaa              >  2:323989/1‑139 (MQ=255)                                                                                                                  aaCGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat       <  1:323989/139‑1 (MQ=255)                                                                                                                       gCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACca  <  2:500655/139‑1 (MQ=255)                                                                                                                       gCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACca  <  1:538336/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                GTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCA  >  NZ_CP009273/3050378‑3050634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑