Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,063,057	A→T	I37N (ATC→AAC)	mscS ←	small‑conductance mechanosensitive channel MscS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,063,057	0	A	T	100.0%	220.9 / NA	55	I37N (ATC→AAC)	mscS	small‑conductance mechanosensitive channel MscS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/32);  total (23/32)

AGCGTAAAGGCGATAATACCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGATCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCATTCAATATTCCTTTTCAACTGACTCAA  >  NZ_CP009273/3062922‑3063192                                                                                                                                        |                                                                                                                                        aGCGTAAAGGCGATAATACCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTcg                                                                                                                                      >  2:696075/1‑139 (MQ=255)  gCGTAAAGGCGATAATACCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTcgc                                                                                                                                     <  1:250910/139‑1 (MQ=255)        aGGCGATAATACCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTgc                                                                                                                               >  2:566912/1‑139 (MQ=255)        aGGCGATAATACCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTgc                                                                                                                               <  1:696075/139‑1 (MQ=255)             ataataCCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCa                                                                                                                          >  2:345337/1‑139 (MQ=255)              taataCCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCAc                                                                                                                         <  2:812843/139‑1 (MQ=255)                ataCCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACg                                                                                                                        <  1:437730/138‑1 (MQ=255)                      tAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTa                                                                                                                 <  2:334102/139‑1 (MQ=255)                      tAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTa                                                                                                                 >  1:234922/1‑139 (MQ=255)                      tAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTa                                                                                                                 <  1:345337/139‑1 (MQ=255)                      tAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTa                                                                                                                 <  1:411762/139‑1 (MQ=255)                      gaaaggACAAAGGGAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGAACAAAAGGAGAAACAAAGCGTTGGAAATCATCCGCGCGAGAAGAAAAACAACGATGATGTGGGAGAGACCCGCCACGATGTTTa                                                                                                                 >  2:826030/5‑139 (MQ=255)                           gACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCa                                                                                                            <  2:373738/139‑1 (MQ=255)                           gACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCa                                                                                                            <  2:238761/139‑1 (MQ=255)                            accaaTGCAGAAAGAAAATCAGCAACAGCGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCAt                                                                                                           <  1:284995/136‑1 (MQ=255)                            aCTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCa                                                                                                            >  2:467778/1‑138 (MQ=255)                            aCTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCAt                                                                                                           <  2:277547/139‑1 (MQ=255)                             cTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCAt                                                                                                           >  1:128769/1‑138 (MQ=255)                                aTGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTa                                                                                                                 <  2:262773/129‑1 (MQ=255)                                aTGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTa                                                                                                                 >  1:262773/1‑129 (MQ=255)                                      aaagaaaATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCAt                                                                                                           >  2:837518/1‑129 (MQ=255)                                      aaagaaaATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCAt                                                                                                           <  1:837518/129‑1 (MQ=255)                                      aaagaaaATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTagcag                                                                                                 >  1:645128/1‑139 (MQ=255)                                        agaaaATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTagcag                                                                                                 >  1:297703/1‑137 (MQ=255)                                                 gCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGt                                                                                      <  1:467778/139‑1 (MQ=255)                                                  cAACAGAGGGAGCGATTTTACGGTAGAACATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAGCGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGtt                                                                                     <  2:577440/139‑1 (MQ=255)                                                       gTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTa                                                                                <  1:527225/139‑1 (MQ=255)                                                          gCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGtt                                                                                     >  1:687094/1‑131 (MQ=255)                                                          gCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGtt                                                                                     <  2:687094/131‑1 (MQ=255)                                                            aTCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTAccagc                                                                           <  1:462734/139‑1 (MQ=255)                                                                       gggAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCcgc                                                                <  2:750999/139‑1 (MQ=255)                                                                        ggAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCcgcg                                                               <  2:645128/139‑1 (MQ=255)                                                                        ggAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCcgcg                                                               <  1:419604/139‑1 (MQ=255)                                                                        ggAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCcgcg                                                               <  1:566912/139‑1 (MQ=255)                                                                                   ggCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTagcagcagc                                                                                             <  2:176063/98‑1 (MQ=255)                                                                                   ggCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTagcagcagc                                                                                             >  1:176063/1‑98 (MQ=255)                                                                                       aTTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATc                                                 >  1:165047/1‑138 (MQ=255)                                                                                        ttCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACg                                                                                                                        <  2:458284/66‑1 (MQ=255)                                                                                        ttCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACg                                                                                                                        >  1:458284/1‑66 (MQ=255)                                                                                        ttCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGa                                               >  2:287172/1‑139 (MQ=255)                                                                                        ttCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGa                                               >  2:133488/1‑139 (MQ=255)                                                                                          cACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGac                                              <  1:120566/138‑1 (MQ=255)                                                                                           aCCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGAGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCGATCGacaa                                            >  2:97537/1‑139 (MQ=255)                                                                                              gcgTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACAt                                         >  2:100424/1‑139 (MQ=255)                                                                                               cgTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACAtt                                        <  1:652794/139‑1 (MQ=255)                                                                                                          atcCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGaca                                             >  2:606928/1‑123 (MQ=255)                                                                                                          atcCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGaca                                             <  1:606928/123‑1 (MQ=255)                                                                                                          atcCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCa                             <  1:41757/139‑1 (MQ=255)                                                                                                          atcCGCGCGATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCa                             <  1:100424/139‑1 (MQ=255)                                                                                                                  gtnnnncAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCATTCAatat                     <  2:128769/133‑1 (MQ=255)                                                                                                                  gATAATCAAACCAACGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCATTCAatat                     <  2:548053/139‑1 (MQ=255)                                                                                                                              aaCGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACACTCAAATCTTCCATTCAATATTCCTTTTCAACt         >  2:600663/1‑139 (MQ=255)                                                                                                                                cGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGtt                                                                                     >  2:664456/1‑61 (MQ=255)                                                                                                                                cGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGtt                                                                                     <  1:664456/61‑1 (MQ=255)                                                                                                                                cGATGATGTTCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCACCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCATTCAATATTCCTTTTCAACTGa       >  2:840443/1‑139 (MQ=255)                                                                                                                                     atgttCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCATTCAATATTCCTTTTCAACTGACTCaa  <  1:145155/139‑1 (MQ=255)                                                                                                                                     atgttCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCATTCAATATTCCTTTTCAACTGACTCa   >  2:197864/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                        AGCGTAAAGGCGATAATACCGTAACGGACTAATGCAGAAAGAAAATCAGCAACAGTGGCATCGATTTTACGGGAGATCATCAGGCGATTCACCGCGTTGGAAATCATCCGCGCGATAATCAAACCAACGATGATGATCGCGAGTGCCGCCACGATGTTTACTGCATAACTTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCTTCCATTCAATATTCCTTTTCAACTGACTCAA  >  NZ_CP009273/3062922‑3063192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑