Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,168,157	A→T	I200N (ATC→AAC)	parE ←	DNA topoisomerase IV subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,168,157	0	A	T	100.0%	124.0 / NA	32	I200N (ATC→AAC)	parE	DNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/15);  total (17/15)

ACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTG  >  NZ_CP009273/3168024‑3168279                                                                                                                                      |                                                                                                                           aCCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTtctc                                                                                                                        >  2:618439/1‑138 (MQ=255)       gAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGc                                                                                                                   >  1:106959/1‑137 (MQ=255)       gAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCa                                                                                                                 <  2:501694/139‑1 (MQ=255)       gAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCa                                                                                                                 <  1:618439/139‑1 (MQ=255)                                     cGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGca                                                                                     >  1:533797/1‑137 (MQ=255)                                     cGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGca                                                                                     <  2:533797/137‑1 (MQ=255)                                           cAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTc                                                                             <  1:693079/139‑1 (MQ=255)                                               ccATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCa                                                                                                                 >  2:830939/1‑99 (MQ=255)                                               ccATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCa                                                                                                                 <  1:830939/99‑1 (MQ=255)                                                  tttACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGcaca                                                                                   >  1:356239/1‑126 (MQ=255)                                                  tttACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGcaca                                                                                   <  2:356239/126‑1 (MQ=255)                                                          ttCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGa                                                               >  1:166875/1‑138 (MQ=255)                                                                   gTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg                                                     <  1:120049/139‑1 (MQ=255)                                                                             aGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAaag                                            >  2:722622/1‑138 (MQ=255)                                                                                   tCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGttt                                     >  1:382836/1‑139 (MQ=255)                                                                                   tCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGttt                                     >  2:88806/1‑139 (MQ=255)                                                                                   tCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGttt                                     >  1:713458/1‑139 (MQ=255)                                                                                    ccTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTg                                                                      >  1:310937/1‑105 (MQ=255)                                                                                    ccTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTg                                                                      <  2:310937/105‑1 (MQ=255)                                                                                             aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGca                                                                                     <  2:89885/81‑1 (MQ=255)                                                                                             aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGca                                                                                     >  1:89885/1‑81 (MQ=255)                                                                                             aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc                             >  2:745968/1‑137 (MQ=255)                                                                                              ccAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAg                          <  1:33612/139‑1 (MQ=255)                                                                                                    ttgttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGc                    <  2:713458/139‑1 (MQ=255)                                                                                                       ttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGcaca                 >  2:246517/1‑139 (MQ=255)                                                                                                       ttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGcac                  <  2:162865/138‑1 (MQ=255)                                                                                                          ggTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACt               <  1:246517/138‑1 (MQ=255)                                                                                                            tATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTgg             >  1:381110/1‑138 (MQ=255)                                                                                                              ttgttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTAc          <  2:791559/139‑1 (MQ=255)                                                                                                                      ctcATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg  >  1:685841/1‑139 (MQ=255)                                                                                                                         aTCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg                                                     <  2:104043/85‑1 (MQ=255)                                                                                                                         aTCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg                                                     >  1:104043/1‑85 (MQ=255)                                                                                                                                      |                                                                                                                           ACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTG  >  NZ_CP009273/3168024‑3168279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑