Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,223,174	A→T	I310F (ATC→TTC)	ygjK →	alpha‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,223,174	0	A	T	100.0%	194.0 / NA	51	I310F (ATC→TTC)	ygjK	alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/24);  total (27/24)

GATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGA  >  NZ_CP009273/3223044‑3223309                                                                                                                                   |                                                                                                                                        gATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACg                                                                                                                                 >  2:684940/1‑139 (MQ=255)   tCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCt                                                                                                                               <  1:373079/139‑1 (MQ=255)              cTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAAc                                                                                                                     <  2:94698/138‑1 (MQ=255)               tGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTg                                                                                                                   >  1:605609/1‑139 (MQ=255)                 gcgcGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGgc                                                                                                                 <  1:204109/139‑1 (MQ=255)                    cgTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCt                                                                                                              >  2:375086/1‑139 (MQ=255)                     gTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTc                                                                                                             <  1:684940/139‑1 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGTAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCa                                                                                                            >  1:417748/1‑139 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCg                                                                                                                                      <  1:49367/113‑1 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCg                                                                                                                                      >  2:49367/1‑113 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCa                                                                                                            >  2:33304/1‑139 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCa                                                                                                            >  1:364414/1‑139 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCa                                                                                                            >  2:693511/1‑139 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCa                                                                                                            >  1:557894/1‑139 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCa                                                                                                            >  2:551625/1‑139 (MQ=255)                      tCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGCTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCa                                                                                                            >  2:161403/1‑139 (MQ=255)                           cGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCggg                                                                                                       <  2:557894/139‑1 (MQ=255)                                      aCCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGcggtgcggtg                                                                                             <  1:761536/138‑1 (MQ=255)                                           cTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAAttt                                                                                       <  1:161403/139‑1 (MQ=255)                                              gcagcaACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTaa                                                                                     <  1:63340/138‑1 (MQ=255)                                              gcagcaACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTaa                                                                                     <  2:605609/138‑1 (MQ=255)                                              gcagcaACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTaa                                                                                     <  2:417748/138‑1 (MQ=255)                                              gcagcaACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTaa                                                                                     <  1:375086/138‑1 (MQ=255)                                                  caACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCg                                                                                >  2:681326/1‑139 (MQ=255)                                                     cGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTaa                                                                                     >  1:699063/1‑131 (MQ=255)                                                     cGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTaa                                                                                     <  2:699063/131‑1 (MQ=255)                                                     cGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTa                                                                             >  1:588962/1‑139 (MQ=255)                                                       cTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTaca                                                                           <  2:314152/139‑1 (MQ=255)                                                          ggAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCg                                                                        >  1:25576/1‑139 (MQ=255)                                                               aaTACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGt                                                                       <  1:724255/135‑1 (MQ=255)                                                               aaTACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGt                                                                       >  2:724255/1‑135 (MQ=255)                                                                  aCCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGAc                                                                >  2:31555/1‑139 (MQ=255)                                                                    cTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCg                                                              >  2:386244/1‑139 (MQ=255)                                                                      gaagaaAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCggg                                                            >  2:325580/1‑139 (MQ=255)                                                                         gaaAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGgcgc                                                         >  2:141052/1‑139 (MQ=255)                                                                                 tAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTtctc                                                 >  2:49987/1‑139 (MQ=255)                                                                                  aaCCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTcc                                                >  2:534153/1‑139 (MQ=255)                                                                                   aCCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAATGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCg                                               <  1:27742/139‑1 (MQ=255)                                                                                        tCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCggg                                                                                                       >  1:546790/1‑78 (MQ=255)                                                                                        tCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCggg                                                                                                       <  2:546790/78‑1 (MQ=255)                                                                                        tCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAAt                                          <  2:525153/139‑1 (MQ=255)                                                                                        tCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAAt                                          >  1:217449/1‑139 (MQ=255)                                                                                        tCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAAt                                          >  2:20527/1‑139 (MQ=255)                                                                                              tGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGAcc                                    <  1:693511/139‑1 (MQ=255)                                                                                              tGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGAcc                                    >  2:348708/1‑139 (MQ=255)                                                                                                        gAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTggg                          <  1:286852/139‑1 (MQ=255)                                                                                                        gAACAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTggg                          <  1:459130/139‑1 (MQ=255)                                                                                                         aaCAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGa                         <  1:348708/139‑1 (MQ=255)                                                                                                         aaCAGACGCGCGTCGCGGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGa                         <  1:551625/139‑1 (MQ=255)                                                                                                                        cgGTGAAAGCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGc          >  2:529253/1‑139 (MQ=255)                                                                                                                                gCGTTCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGa  <  2:217449/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGA  >  NZ_CP009273/3223044‑3223309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑