Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,403,270	A→G	E281G (GAA→GGA)	prmA →	50S ribosomal protein L11 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,403,270	0	A	G	100.0%	137.5 / NA	38	E281G (GAA→GGA)	prmA	50S ribosomal protein L11 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

TTACGTGAACTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGAAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTAC  >  NZ_CP009273/3403134‑3403389                                                                                                                                         |                                                                                                                        ttACGTGAACTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGgaa                                                                                                                       <  2:589055/139‑1 (MQ=255)        aaCTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAaagaa                                                                                                                  >  1:284943/1‑137 (MQ=255)         aCTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAagag                                                                                                               >  2:830317/1‑139 (MQ=255)             gCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtgg                                                                                                            <  2:284943/138‑1 (MQ=255)             gCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtgg                                                                                                            <  1:616622/138‑1 (MQ=255)              cACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggtg                                                                                                          <  1:349584/139‑1 (MQ=255)              cACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggtg                                                                                                          <  1:830317/139‑1 (MQ=255)              cACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggt                                                                                                           >  2:75371/1‑138 (MQ=255)              cACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggt                                                                                                           <  1:75371/138‑1 (MQ=255)                ccGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGcc                                                                                                        <  2:270065/139‑1 (MQ=255)                   ttAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTa                                                                                                     >  1:382883/1‑139 (MQ=255)                         aGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTAcc                                                                                                <  2:714839/138‑1 (MQ=255)                           cGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGt                                                                                             >  1:284247/1‑139 (MQ=255)                             tCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAagag                                                                                                               >  2:607050/1‑119 (MQ=255)                             tCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAagag                                                                                                               <  1:607050/119‑1 (MQ=255)                                tGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGt                                                                                          >  2:466136/1‑137 (MQ=255)                                tGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGt                                                                                          >  2:212186/1‑137 (MQ=255)                                   cGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGaa                                                                                     >  1:267367/1‑139 (MQ=255)                                      tttCAGGCGGTTTGCTGGGCCTTTCCGGTATTCT‑‑‑‑‑‑‑‑‑GGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa                                                                         <  1:372795/139‑1 (MQ=255)                                                         ccTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAgg                                                               <  2:344120/139‑1 (MQ=255)                                                             tCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGa                                                           >  1:177710/1‑139 (MQ=255)                                                               cGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGAcg                                                         <  1:691975/139‑1 (MQ=255)                                                                ggTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGAcgc                                                        <  2:370503/139‑1 (MQ=255)                                                                 gTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGAcgcg                                                       >  2:756752/1‑139 (MQ=255)                                                                         gCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCg                                                  <  2:267367/136‑1 (MQ=255)                                                                         gCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAg                                               >  2:155082/1‑139 (MQ=255)                                                                         gCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAg                                               >  2:772076/1‑139 (MQ=255)                                                                              ccAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggtg                                                                                                          >  2:691194/1‑75 (MQ=255)                                                                              ccAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggtg                                                                                                          <  1:691194/75‑1 (MQ=255)                                                                                       gagCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAg                                     <  1:27477/135‑1 (MQ=255)                                                                                       gagCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAg                                     >  2:27477/1‑135 (MQ=255)                                                                                        agCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGAcgc                                                        >  2:566605/1‑115 (MQ=255)                                                                                        agCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGAcgc                                                        <  1:566605/115‑1 (MQ=255)                                                                                            tttGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa                                                                         >  2:372158/1‑94 (MQ=255)                                                                                            tttGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa                                                                         <  1:372158/94‑1 (MQ=255)                                                                                                  aaGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACg                      >  1:738527/1‑139 (MQ=255)                                                                                                              aTAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTAcc                                                                                                <  2:390550/53‑1 (MQ=255)                                                                                                              aTAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTAcc                                                                                                >  1:390550/1‑53 (MQ=255)                                                                                                                      gCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTAc  <  1:466136/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                        TTACGTGAACTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGAAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTAC  >  NZ_CP009273/3403134‑3403389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑