Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,429,447	T→G	G294G (GGT→GGG)	rsmB →	16S rRNA (cytosine(967)‑C(5))‑methyltransferase RsmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,429,447	0	T	G	100.0%	119.4 / NA	31	G294G (GGT→GGG)	rsmB	16S rRNA (cytosine(967)‑C(5))‑methyltransferase RsmB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/14);  total (17/14)

TTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTA  >  NZ_CP009273/3429323‑3429577                                                                                                                             |                                                                                                                                   tttGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGt                                                                                                                      >  1:33976/1‑139 (MQ=255) tttGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGt                                                                                                                      >  1:562103/1‑139 (MQ=255)       ccgccCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAAc                                                                                                                 <  2:789987/138‑1 (MQ=255)                      aaaCAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTa                                                                                                 <  1:362836/139‑1 (MQ=255)                      aaaCAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTa                                                                                                 <  1:51120/139‑1 (MQ=255)                           acGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGGGATGGCCGTTACCCtt                                                                                            >  2:582353/1‑139 (MQ=255)                               atatCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCa                                                                                        <  2:394581/139‑1 (MQ=255)                                tatCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCaa                                                                                       <  2:33976/139‑1 (MQ=255)                                      tGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGgtgt                                                                                 >  2:691862/1‑139 (MQ=255)                                       gAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGtgtg                                                                                >  1:379297/1‑139 (MQ=255)                                         ggTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGc                                                                              <  2:562103/139‑1 (MQ=255)                                             gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                                                           >  2:444321/1‑138 (MQ=255)                                             gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                                                           >  2:246568/1‑138 (MQ=255)                                             gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                                                           >  2:794216/1‑138 (MQ=255)                                                        gcAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGAt                                                               <  2:665471/139‑1 (MQ=255)                                                         cAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATc                                                              >  2:567721/1‑139 (MQ=255)                                                                   cGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTttat                                                     <  1:627471/138‑1 (MQ=255)                                                                     gTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAg                                                  <  2:633394/139‑1 (MQ=255)                                                                      ttGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTAcc                                                                                               >  2:252123/1‑93 (MQ=255)                                                                      ttGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTAcc                                                                                               <  1:252123/93‑1 (MQ=255)                                                                           atCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATgcgc                                            >  2:115625/1‑139 (MQ=255)                                                                                    cAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTa                                                                                                 <  2:682489/77‑1 (MQ=255)                                                                                    cAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTa                                                                                                 >  1:682489/1‑77 (MQ=255)                                                                                             tctcGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGgtg                          >  2:102652/1‑139 (MQ=255)                                                                                                        cGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATtcgtcg                <  2:576481/138‑1 (MQ=255)                                                                                                             aTTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATc           >  2:470337/1‑138 (MQ=255)                                                                                                              tttAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATc           >  2:502219/1‑137 (MQ=255)                                                                                                              tttAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATc           >  1:713882/1‑137 (MQ=255)                                                                                                                     cGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTa  <  1:39455/139‑1 (MQ=255)                                                                                                                        cTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCAtt                                                         >  1:104106/1‑81 (MQ=255)                                                                                                                        cTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCAtt                                                         <  2:104106/81‑1 (MQ=255)                                                                                                                             |                                                                                                                                   TTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTA  >  NZ_CP009273/3429323‑3429577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑