Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,501,576	T→C	E125G (GAG→GGG)	php ←	phosphotriesterase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,501,576	0	T	C	100.0%	187.0 / NA	52	E125G (GAG→GGG)	php	phosphotriesterase‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/28);  total (24/28)

CAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTA  >  NZ_CP009273/3501452‑3501710                                                                                                                             |                                                                                                                                       cAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCg                                                                                                                          <  1:597244/139‑1 (MQ=255) cAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCg                                                                                                                          <  1:277366/139‑1 (MQ=255)  aGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCgg                                                                                                                         >  2:268304/1‑139 (MQ=255)             gCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTcc                                                                                                              >  1:514546/1‑139 (MQ=255)                gAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTg                                                                                                           >  1:657729/1‑139 (MQ=255)                 aaCGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGt                                                                                                            <  2:254680/137‑1 (MQ=255)                 aaCGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          <  2:455584/139‑1 (MQ=255)                 aaCGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          <  1:67949/139‑1 (MQ=255)                 aaCGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          <  2:657729/139‑1 (MQ=255)                      cgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATc                                                                                                      >  1:110758/1‑138 (MQ=255)                           gCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATAcc                                                                                                >  1:426559/1‑139 (MQ=255)                           gCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATAcc                                                                                                >  1:766864/1‑139 (MQ=255)                           gCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATAcc                                                                                                >  2:356520/1‑139 (MQ=255)                                gagaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          >  1:364923/1‑124 (MQ=255)                                gagaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          <  2:364923/124‑1 (MQ=255)                                gagaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGtt                                                                                           >  2:598305/1‑139 (MQ=255)                                  gaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCg                                                                                                                          <  2:61741/106‑1 (MQ=255)                                  gaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCg                                                                                                                          >  1:61741/1‑106 (MQ=255)                                       ggCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAttt                                                                                     >  2:207790/1‑138 (MQ=255)                                       ggCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAttt                                                                                     >  2:399519/1‑138 (MQ=255)                                       ggCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAttt                                                                                     >  2:58385/1‑138 (MQ=255)                                         cGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCAt                                                                                  <  2:766864/139‑1 (MQ=255)                                         cGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCAt                                                                                  <  2:330027/139‑1 (MQ=255)                                         cGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCAt                                                                                  >  2:562043/1‑139 (MQ=255)                                           tCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCg                                                                                <  2:487992/139‑1 (MQ=255)                                              ggTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGAcc                                                                             <  1:172960/139‑1 (MQ=255)                                                         gcgcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          >  1:567047/1‑99 (MQ=255)                                                         gcgcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          <  2:567047/99‑1 (MQ=255)                                                          cgcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTgcgc                                                                  >  1:27393/1‑138 (MQ=255)                                                          cgcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTgcgc                                                                  <  1:207790/138‑1 (MQ=255)                                                          cgcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGcc                                                                 <  2:35984/139‑1 (MQ=255)                                                              agcgcagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGtt                                                             >  2:220932/1‑139 (MQ=255)                                                               gcgcagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTc                                                            <  1:13145/139‑1 (MQ=255)                                                                   agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGc                                                        <  1:220932/139‑1 (MQ=255)                                                                    gcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCa                                                       <  2:766299/139‑1 (MQ=255)                                                                    gcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCa                                                       <  1:455155/139‑1 (MQ=255)                                                                     cgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCAc                                                      >  1:318262/1‑139 (MQ=255)                                                                           taaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCg                                                <  2:110758/139‑1 (MQ=255)                                                                                    tctcTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          <  2:1559/72‑1 (MQ=255)                                                                                    tctcTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                          >  1:1559/1‑72 (MQ=255)                                                                                    tctcTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCca                                        >  1:75552/1‑138 (MQ=255)                                                                                        ttCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGt                                   >  1:602694/1‑139 (MQ=255)                                                                                        ttCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGt                                   >  1:54597/1‑139 (MQ=255)                                                                                           cAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTc                                 <  2:602694/138‑1 (MQ=255)                                                                                                   tAATCTTTCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaaaaa                        >  1:342705/1‑139 (MQ=255)                                                                                                          tCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTc                  <  2:514546/138‑1 (MQ=255)                                                                                                          tCCTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTc                  <  1:268304/138‑1 (MQ=255)                                                                                                           ccTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTcc                 <  2:75552/138‑1 (MQ=255)                                                                                                           ccTTCGCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCt                >  1:37110/1‑139 (MQ=255)                                                                                                                gCTGGTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGta            <  2:838358/138‑1 (MQ=255)                                                                                                                    gTGCCGATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAAc       <  2:342705/139‑1 (MQ=255)                                                                                                                         gATCCCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCTAGACCCTGTATAATTGCATCCACCATCTCCTACCCCACTTCCTTCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTa  <  2:41217/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                       CAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTA  >  NZ_CP009273/3501452‑3501710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑