Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,539,325 C→T L115L (CTG→TTG)  nfuA → Fe‑S biogenesis protein NfuA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,539,3250CT90.2% 130.0 / 5.3 41L115L (CTG→TTG) nfuAFe‑S biogenesis protein NfuA
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (19/18);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

CGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTG  >  NZ_CP009273/3539189‑3539459
                                                                                                                                        |                                                                                                                                      
cgcACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgttg                                                                                                                                      <  1:473158/139‑1 (MQ=255)
cgcACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgttg                                                                                                                                      <  2:491131/139‑1 (MQ=255)
     cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTc                                                                                                                                 >  2:844428/1‑139 (MQ=255)
     cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTc                                                                                                                                 >  1:373173/1‑139 (MQ=255)
     cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTc                                                                                                                                 >  2:830645/1‑139 (MQ=255)
         ccTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCa                                                                                                                              <  2:373173/138‑1 (MQ=255)
             gAAGATGCAGATATCGATTTTGTTCCCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGAt                                                                                                                           <  1:420182/137‑1 (MQ=255)
                gATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAAcc                                                                                                                      <  1:354367/139‑1 (MQ=255)
                  tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgttg                                                                                                                                      <  1:374197/121‑1 (MQ=255)
                  tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgttg                                                                                                                                      >  2:374197/1‑121 (MQ=255)
                  tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca                                                                                                                    >  2:839769/1‑139 (MQ=255)
                  tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca                                                                                                                    >  1:290909/1‑139 (MQ=255)
                  tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca                                                                                                                    >  2:37932/1‑139 (MQ=255)
                  tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca                                                                                                                    >  1:655998/1‑139 (MQ=255)
                   gCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca                                                                                                                    >  2:423367/1‑138 (MQ=255)
                    cAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCcaca                                                                                                                  <  1:37932/139‑1 (MQ=255)
                        gaTCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCtt                                                                                                              >  1:804007/1‑139 (MQ=255)
                                   ttACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACgg                                                                                                   >  2:748931/1‑139 (MQ=255)
                                                         gTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGaa                                                                             <  2:804007/139‑1 (MQ=255)
                                                                   aaaGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGaa                                                                    <  2:348108/138‑1 (MQ=255)
                                                                            aaCGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACg                                                          <  2:811905/139‑1 (MQ=255)
                                                                             aCGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGc                                                         >  2:186151/1‑139 (MQ=255)
                                                                               gCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCa                                                       >  1:168816/1‑139 (MQ=255)
                                                                                                  cAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTc                                                                                                                                 >  2:524474/1‑46 (MQ=255)
                                                                                                  cAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTc                                                                                                                                 <  1:524474/46‑1 (MQ=255)
                                                                                                  cAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggcgg                                    >  2:107611/1‑139 (MQ=255)
                                                                                                      cGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGt                                <  1:748931/139‑1 (MQ=255)
                                                                                                        aTGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              <  1:423367/139‑1 (MQ=255)
                                                                                                        aTGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              <  2:553319/139‑1 (MQ=255)
                                                                                                         tGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAAc                             >  2:17140/1‑139 (MQ=255)
                                                                                                           cACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACgg                           >  1:829505/1‑139 (MQ=255)
                                                                                                                cTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgttgtt                      <  1:810604/139‑1 (MQ=255)
                                                                                                                    tGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCAt                  <  1:839769/139‑1 (MQ=255)
                                                                                                                       aGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGt               <  2:829505/139‑1 (MQ=255)
                                                                                                                           cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              <  2:237395/120‑1 (MQ=255)
                                                                                                                           cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              >  2:195718/1‑120 (MQ=255)
                                                                                                                           cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              >  1:825978/1‑120 (MQ=255)
                                                                                                                           cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              <  2:825978/120‑1 (MQ=255)
                                                                                                                           cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              >  1:237395/1‑120 (MQ=255)
                                                                                                                           cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa                              <  1:195718/120‑1 (MQ=255)
                                                                                                                           cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAt           >  1:533572/1‑139 (MQ=255)
                                                                                                                                  tataTGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGc    <  1:595964/139‑1 (MQ=255)
                                                                                                                                    tatGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTg  <  1:830645/139‑1 (MQ=255)
                                                                                                                                     atGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgttgtt                      <  2:730665/118‑1 (MQ=255)
                                                                                                                                     atGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgttgtt                      >  1:730665/1‑118 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTG  >  NZ_CP009273/3539189‑3539459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑