Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,464,092	(C)6→5	coding (152/594 nt)	bdcR →	TetR/AcrR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,464,087	0	C	.	100.0%	165.5 / NA	38	coding (147/594 nt)	bdcR	TetR/AcrR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (17/21);  total (17/21)

AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGT  >  NZ_CP009273/4463954‑4464214                                                                                                                                      |                                                                                                                                aaCAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAAcccccg                                                                                                                            <  1:70170/139‑2 (MQ=255)      tctcGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCct                                                                                                                      >  2:137762/1‑139 (MQ=255)                       tcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTgg                                                                                                       >  2:184700/1‑137 (MQ=255)                       tcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTgg                                                                                                       >  2:118851/1‑137 (MQ=255)                       tcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTgg                                                                                                       >  1:407509/1‑137 (MQ=255)                        cccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAg                                                                                                    >  2:137098/1‑139 (MQ=255)                        cccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAg                                                                                                    >  2:394711/1‑139 (MQ=255)                        cccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGAGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAg                                                                                                    >  1:91100/1‑139 (MQ=255)                                 ttCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTgg                                                                                           >  1:345085/1‑139 (MQ=255)                                    gCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGtt                                                                                        <  1:447574/139‑1 (MQ=255)                                        cTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTAtt                                                                                     <  1:363380/138‑1 (MQ=255)                                        cTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTAtt                                                                                     <  2:454831/138‑1 (MQ=255)                                        cTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTAtt                                                                                     <  1:312224/138‑1 (MQ=255)                                            gCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGcc                                                                                <  1:351111/139‑1 (MQ=255)                                                 cAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTa                                                                           <  1:394711/139‑1 (MQ=255)                                                 cAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTa                                                                           <  2:469318/139‑1 (MQ=255)                                                 cAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTa                                                                           <  1:293506/139‑1 (MQ=255)                                                          cGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGaa                                                                  >  2:118665/1‑139 (MQ=255)                                                             cAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATAc                                                               <  2:320370/139‑1 (MQ=255)                                                               aaaGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGt                                                             <  1:283739/139‑1 (MQ=255)                                                                     cTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTAc                                                       <  2:345085/139‑1 (MQ=255)                                                                        tttCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACg                                                      <  2:165976/137‑1 (MQ=255)                                                                         ttCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGa                                                    <  1:306418/138‑1 (MQ=255)                                                                                    ggTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCt                                        <  1:275792/139‑1 (MQ=255)                                                                                    ggTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCt                                        <  1:236434/139‑1 (MQ=255)                                                                                    ggTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCt                                        <  2:91100/139‑1 (MQ=255)                                                                                                 tCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATAttc                             <  1:398004/137‑1 (MQ=255)                                                                                                        tGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGtgat                     >  2:304178/1‑138 (MQ=255)                                                                                                         gCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGtgatg                    <  1:324340/138‑1 (MQ=255)                                                                                                          cTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTgatgat                  <  1:118851/139‑1 (MQ=255)                                                                                                          cTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTgatgat                  >  2:94788/1‑139 (MQ=255)                                                                                                           tGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATc                 >  1:348278/1‑139 (MQ=255)                                                                                                                         aTCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAg   >  2:186531/1‑139 (MQ=255)                                                                                                                         aTCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAg   >  1:190759/1‑139 (MQ=255)                                                                                                                          tCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGt  >  1:73054/1‑139 (MQ=255)                                                                                                                              ggTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGt                                                             <  2:294356/76‑1 (MQ=255)                                                                                                                              ggTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGt                                                             <  2:225148/76‑1 (MQ=255)                                                                                                                              ggTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGt                                                             >  1:294356/1‑76 (MQ=255)                                                                                                                              ggTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGt                                                             >  1:225148/1‑76 (MQ=255)                                                                                                                                      |                                                                                                                                AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGT  >  NZ_CP009273/4463954‑4464214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑