| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,188,709 | (T)5→4 | coding (521/1107 nt) | mnmA ← | tRNA 2‑thiouridine(34) synthase MnmA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,188,705 | 0 | T | . | 100.0% | 66.3 / NA | 16 | coding (525/1107 nt) | mnmA | tRNA 2‑thiouridine(34) synthase MnmA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (6/10); total (6/10) | |||||||||||
TTGCGCCGGGAGATAACGGCCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGCCC > NZ_CP009273/1188579‑1188835 | ttGCGCCGGGAGATAACGGCCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGc < 1:209203/139‑1 (MQ=255) gcCGGGAGATAACGGCCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGAc < 1:433572/139‑1 (MQ=255) aCGGCCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAgg < 2:272495/138‑1 (MQ=255) aaCTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTGCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAAt < 2:109174/139‑1 (MQ=255) gcgGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGc < 1:197992/138‑1 (MQ=255) aTTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATgg < 1:2171/139‑1 (MQ=255) cGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGa < 1:265286/138‑1 (MQ=255) aTGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGct < 1:345227/139‑1 (MQ=255) ttttttCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGc > 2:221244/1‑139 (MQ=255) tcGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGa > 1:44851/1‑139 (MQ=255) gATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGctc > 1:400581/1‑139 (MQ=255) gATCTTCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGctc > 2:174007/1‑139 (MQ=255) tCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTgcg < 1:106464/57‑1 (MQ=255) tCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTgcg > 2:106464/1‑57 (MQ=255) tCAGCAATCTTACGCACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGc > 2:204443/1‑138 (MQ=255) cACCTGCGG‑TTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGccc < 1:154170/139‑1 (MQ=255) | TTGCGCCGGGAGATAACGGCCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGCCC > NZ_CP009273/1188579‑1188835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |