Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,974,914	Δ1 bp	coding (628/1419 nt)	araE ←	arabinose‑proton symporter AraE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,974,914	0	G	.	100.0%	138.4 / NA	32	coding (628/1419 nt)	araE	arabinose‑proton symporter AraE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (17/15);  total (17/15)

TTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAA  >  NZ_CP009273/2974781‑2975047                                                                                                                                      |                                                                                                                                      ttACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCtt                                                                                                                                 <  1:115870/139‑1 (MQ=255)                     tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGcgcg                                                                                                                >  2:98973/1‑136 (MQ=255)                     tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGcgcg                                                                                                                <  1:98973/136‑1 (MQ=255)                     tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:437658/1‑139 (MQ=255)                     tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:366224/1‑139 (MQ=255)                     tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:281041/1‑139 (MQ=255)                     tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  1:72641/1‑139 (MQ=255)                     tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  1:61588/1‑139 (MQ=255)                               cccTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAg                                                                                                   >  1:146389/1‑139 (MQ=255)                               cccTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAg                                                                                                   >  1:383504/1‑139 (MQ=255)                               cccTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAg                                                                                                   >  1:207702/1‑139 (MQ=255)                                    ttttAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa                                                                                                <  2:146389/137‑1 (MQ=255)                                        aaCTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTa                                                                                           <  1:330186/138‑1 (MQ=255)                                              aGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaa                                                                                     >  2:211883/1‑138 (MQ=255)                                                     cACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATcag                                                                              >  1:207784/1‑138 (MQ=255)                                                                        ttctCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGc                                                            >  2:496123/1‑137 (MQ=255)                                                                             gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  1:496123/136‑1 (MQ=255)                                                                             gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  2:72641/136‑1 (MQ=255)                                                                             gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  1:366224/136‑1 (MQ=255)                                                                             gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  2:337815/136‑1 (MQ=255)                                                                                                 cgcAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGtt                                 <  2:137416/139‑1 (MQ=255)                                                                                                   cagcaTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTAc                               <  2:207784/139‑1 (MQ=255)                                                                                                                aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAAcccc                                                   <  1:328397/106‑1 (MQ=255)                                                                                                                aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAAcccc                                                   >  2:328397/1‑106 (MQ=255)                                                                                                                aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                  >  2:25996/1‑139 (MQ=255)                                                                                                                aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                  >  1:317602/1‑139 (MQ=255)                                                                                                                 cttcttCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                  <  2:207702/138‑1 (MQ=255)                                                                                                                         gCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaata                                                                                   <  2:394987/65‑1 (MQ=255)                                                                                                                         gCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaata                                                                                   >  1:394987/1‑65 (MQ=255)                                                                                                                         gCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATc         >  2:495548/1‑139 (MQ=255)                                                                                                                                tatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATaaa  <  1:240301/139‑1 (MQ=255)                                                                                                                                tatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATaaa  <  1:281041/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAA  >  NZ_CP009273/2974781‑2975047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑