Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,000,345	(A)5→4	coding (722/1188 nt)	ygeW →	knotted carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,000,341	0	A	.	100.0%	184.3 / NA	42	coding (718/1188 nt)	ygeW	knotted carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (24/18);  total (24/18)

CTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTACTG  >  NZ_CP009273/3000208‑3000467                                                                                                                                      |                                                                                                                               cTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGaaaaca                                                                                                                           <  1:34255/139‑3 (MQ=255)    ctctCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATg                                                                                                                       >  1:448338/1‑139 (MQ=255)    ctctCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATg                                                                                                                       >  1:407627/1‑139 (MQ=255)                 ggCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCgg                                                                                                          >  1:288661/1‑139 (MQ=255)                 ggCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCgg                                                                                                          >  2:271348/1‑139 (MQ=255)                       ccGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTGCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAg                                                                                                    <  2:263389/139‑1 (MQ=255)                          ctctctGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCtt                                                                                                 >  1:472807/1‑139 (MQ=255)                                 tACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTc                                                                                            <  2:297302/137‑1 (MQ=255)                                 tACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAg                                                                                          >  2:218649/1‑139 (MQ=255)                                 tACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAg                                                                                          <  2:448338/139‑1 (MQ=255)                                       aaGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCAc                                                                                     >  1:64386/1‑138 (MQ=255)                                         ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGaaaaca                                                                                                                           >  1:166547/1‑97 (MQ=255)                                         ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGaaaaca                                                                                                                           <  2:166547/99‑3 (MQ=255)                                         ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAg                                                                                  >  1:114770/1‑139 (MQ=255)                                         ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAg                                                                                  >  2:250252/1‑139 (MQ=255)                                         ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAg                                                                                  >  1:449810/1‑139 (MQ=255)                                         ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAg                                                                                  >  2:463354/1‑139 (MQ=255)                                           catcatCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCa                                                                                <  1:39898/139‑1 (MQ=255)                                           catcatCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCa                                                                                >  2:243781/1‑139 (MQ=255)                                              catcGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATg                                                                              >  2:320235/1‑138 (MQ=255)                                                        atgaCTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGaaaa                                                                                                                             <  1:183253/82‑1 (MQ=255)                                                        atgaCTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGaaaa                                                                                                                             >  2:183253/1‑82 (MQ=255)                                                                  tCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCAc                                                                                     <  1:202923/111‑1 (MQ=255)                                                                  tCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCAc                                                                                     >  2:202923/1‑111 (MQ=255)                                                                    ggTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGa                                                       <  1:218649/139‑1 (MQ=255)                                                                                aCCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGAc                                                      >  2:106616/1‑128 (MQ=255)                                                                                aCCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGAc                                                      <  1:106616/128‑1 (MQ=255)                                                                                 cccTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCg                                          <  2:449810/139‑1 (MQ=255)                                                                                   cTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGaa                                        <  2:139894/139‑1 (MQ=255)                                                                                     ggCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             >  2:252409/1‑84 (MQ=255)                                                                                     ggCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             <  1:252409/84‑1 (MQ=255)                                                                                           tCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             >  1:286962/1‑78 (MQ=255)                                                                                           tCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             <  2:286962/78‑1 (MQ=255)                                                                                           tCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATggg                                >  2:421404/1‑139 (MQ=255)                                                                                             cGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGc                               <  2:381760/138‑1 (MQ=255)                                                                                                     tACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACa                       >  2:418675/1‑138 (MQ=255)                                                                                                       cGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACaa                      <  1:463354/137‑1 (MQ=255)                                                                                                             gATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATggg                                >  1:36875/1‑121 (MQ=255)                                                                                                             gATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATggg                                <  2:36875/121‑1 (MQ=255)                                                                                                              aTCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATgg              >  2:480540/1‑138 (MQ=255)                                                                                                                 ccGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAaga          <  1:421404/139‑1 (MQ=255)                                                                                                                 ccGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAaga          <  2:472807/139‑1 (MQ=255)                                                                                                                    gATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCg       <  1:320235/139‑1 (MQ=255)                                                                                                                        tgGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGa                 <  2:497830/125‑1 (MQ=255)                                                                                                                        tgGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACAAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGa                 >  1:497830/1‑125 (MQ=255)                                                                                                                          gTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTACTg  >  2:255011/1‑138 (MQ=255)                                                                                                                          gTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTACTg  >  1:168696/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                               CTACTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTACTG  >  NZ_CP009273/3000208‑3000467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑