Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	297,613	(C)5→6	coding (672/690 nt)	paoA ←	aldehyde dehydrogenase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	297,608	1	.	C	100.0%	78.4 / NA	22	E226G (GAG→GGG)	paoA	aldehyde dehydrogenase iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (13/9);  total (13/9)

TCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑CCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGAC  >  NZ_CP009273/297477‑297735                                                                                                                                     |                                                                                                                                tCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑ccccccg                                                                                                                          >  2:412908/1‑137 (MQ=255)   cAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑ccccccgca                                                                                                                        <  1:420451/139‑5 (MQ=255)   cAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑ccccccgca                                                                                                                        <  1:79108/139‑5 (MQ=255)    aGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑ccccccgcag                                                                                                                       >  1:431305/1‑134 (MQ=255)      cTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCgcagca                                                                                                                      >  1:416082/1‑139 (MQ=255)                tCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCgcagc                                                                                                                       >  1:309342/1‑128 (MQ=255)                tCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCgcagc                                                                                                                       <  2:309342/128‑1 (MQ=255)                      agATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCaa                                                                                                      <  2:416082/139‑1 (MQ=255)                      agATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCaa                                                                                                      <  2:250361/139‑1 (MQ=255)                             tCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGt                                                                                                >  2:82503/1‑138 (MQ=255)                                                         tACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTg                                                                   <  2:24078/139‑1 (MQ=255)                                                         tACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTg                                                                   <  2:61406/139‑1 (MQ=255)                                                                  aGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCa                                                            <  1:464365/137‑1 (MQ=255)                                                                  aGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCAt                                                           <  2:431305/138‑1 (MQ=255)                                                                       tAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGtt                                                      >  1:13441/1‑138 (MQ=255)                                                                         aGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCa                                                    <  1:493602/138‑1 (MQ=255)                                                                                     gCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCgg                                       >  2:306544/1‑139 (MQ=255)                                                                                              ttCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGttt                              >  1:197691/1‑139 (MQ=255)                                                                                                   tCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTg                           >  1:356831/1‑137 (MQ=255)                                                                                                    cGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAg                        >  2:168795/1‑139 (MQ=255)                                                                                                     gTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGc                       >  2:377456/1‑139 (MQ=255)                                                                                                      ttCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCg                      >  2:311440/1‑139 (MQ=255)                                                                                                                aaCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAAt            >  1:323103/1‑139 (MQ=255)                                                                                                                 aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATc           >  1:488542/1‑139 (MQ=255)                                                                                                                 aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATc           >  2:45637/1‑139 (MQ=255)                                                                                                                          tGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTAATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcgtgac  <  2:247444/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                TCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑CCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGAC  >  NZ_CP009273/297477‑297735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑