Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	788,865	+G	coding (380/1473 nt)	modF ←	molybdate ABC transporter ATP‑binding protein ModF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	788,862	1	.	G	100.0%	43.3 / NA	13	L128P (CTC→CCC)	modF	molybdate ABC transporter ATP‑binding protein ModF
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (3/10);  total (3/10)

TCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGA‑GGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATAT  >  NZ_CP009273/788745‑788979                                                                                                                       |                                                                                                                      tCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAActgc                                                                                                   <  1:1772/139‑1 (MQ=255)         aTCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGTTAATACCGAACTGCTGCGCCAg                                                                                           >  2:370249/1‑139 (MQ=255)          tCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGt                                                                                          >  2:232580/1‑139 (MQ=255)           cGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGagga‑gg                                                                                                                     >  1:481607/1‑110 (MQ=255)           cGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGagga‑gg                                                                                                                     <  2:481607/110‑1 (MQ=255)            gAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGtt                                                                                         <  2:138284/138‑1 (MQ=255)                     atcaAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAc                                                                                <  1:232580/138‑1 (MQ=255)                        aaGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcgcg                                                                             <  1:254689/138‑1 (MQ=255)                                          cTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATc                                                          >  2:28734/1‑139 (MQ=255)                                              gACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGa                                                      <  1:380549/139‑1 (MQ=255)                                                     gcgcCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                               <  1:434070/139‑1 (MQ=255)                                                                                                   tttAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCatat  <  1:23963/138‑1 (MQ=255)                                                                                                   tttAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCatat  <  1:28734/138‑1 (MQ=255)                                                                                                   tttAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCatat  <  2:389498/138‑1 (MQ=255)                                                                                                   tttAAAGCGTCGGTCGAGGAGGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCatat  <  2:83453/138‑1 (MQ=255)                                                                                                                       |                                                                                                                      TCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGA‑GGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATAT  >  NZ_CP009273/788745‑788979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑