| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 303,532 | 0 | G | T | 53.8% | 6.5 / 16.5 | 13 | Y504* (TAC→TAA) | ecpC | fimbrial usher EcpC |
| Reads supporting (aligned to +/- strand): ref base G (1/5); new base T (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCA > NZ_CP009273/303399‑303660 | tATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCt < 1:254310/139‑1 (MQ=255) gCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGGCATCATTTTAACTGGTGCTGa > 2:285447/1‑137 (MQ=255) taGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGAcc < 1:368257/139‑1 (MQ=255) cTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTTATCATTTTAGCTTGTGGTGAAATTGCCCCGGTTCcaccaccag > 2:140934/1‑136 (MQ=255) aaTACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGAATGTGCCCAGCTTCGGCCCCAgaggg > 1:85096/1‑135 (MQ=255) gCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTgccgcc < 1:168286/139‑1 (MQ=255) tGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGGATTTGCCCAGCTTCGCCCCCCATGGGTTTAGGTTGTGTGTGCCCCCGATTTCCCGGGTGTCGGc > 1:59672/1‑139 (MQ=255) gTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGGTGCTGAATGTGGCCCGCTTCGGCCACCGTGAGGTCAGGGTGAGGGGGCCGCCGGTTGCACGGGGGTCGGCa > 1:36820/1‑139 (MQ=255) tttGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCCACAATTTGGCTGATGCTGAATGTGGCCAGCGTCGAACACAGGGAGGTGTGGGTGTGTGTGGCGCCGGTTGCGCGGGGGTGGGCGTCGCTACg > 1:65312/1‑139 (MQ=255) gCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGttttt < 1:402156/139‑1 (MQ=255) ggTCATCATTTTAGATGATGCTGAATGGGCCCAGGTTCGACCACAATGGGTTTAGGGTGGGGGGGCCGCCGGGTGTACGGGGGTCGGCCTCGCTACGGCGCAATTGATTGCCAATGCGGGGTTTTTCCTGGTTAACCca > 2:145541/1‑139 (MQ=255) ggTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGtt > 1:432705/1‑52 (MQ=255) ggTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGtt < 2:432705/52‑1 (MQ=255) | TATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCA > NZ_CP009273/303399‑303660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |