Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273360,7620TG62.5% 10.8 / 16.4 16T328P (ACC→CCC) lacIDNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base G (10/0);  total (11/5)
Fisher's exact test for biased strand distribution p-value = 1.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATAT  >  NZ_CP009273/360635‑360899
                                                                                                                               |                                                                                                                                         
aTGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTGTGTGGATTGGGGACCAGGGGGGGTTTTCTTTTCa                                                                                                                                >  1:238272/1‑139 (MQ=255)
     gTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTGGGGACCAGGGGGGGTTTTCTTTTTACCAGt                                                                                                                           >  1:264785/1‑139 (MQ=255)
     gTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGCGGATTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGt                                                                                                                           >  2:121342/1‑139 (MQ=255)
                cgcgTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGTCCAACGCGCGGGGAGAGGCGGTTTTCGTTTTGGGCACCCGGGTGGGTTTTCTTTTCACCCGCGGGACGGGCaa                                                                                                                >  1:190316/1‑139 (MQ=255)
                cgcgTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGACCAGGGGGGGTTTTTTTTTTACCAGGGAGACGGGcca                                                                                                                >  1:183228/1‑137 (MQ=255)
                cgcgTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGCACCAGGGTGGGTTTTCTTTTTACCAGTGAGACGGGcc                                                                                                                 >  1:491610/1‑137 (MQ=255)
                cgcgTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGGACCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCaa                                                                                                                >  1:442293/1‑139 (MQ=255)
                                       tCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGcc                                                                                          <  2:467706/138‑1 (MQ=255)
                                                             ccAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGACCAGGGGGGGTTTTTTTTTCACCCGTGAGACGGGCAACAGCTGATTGGCCTTCACCCCCCGGCCCTGAGAGAGGTgcagca                                                                    >  1:326210/1‑138 (MQ=255)
                                                              cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCCCCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAg                                                                  <  2:342338/139‑1 (MQ=255)
                                                                  tGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGATTGGGGACCAGGGGGGGTTTTCTTTTTACCAGTGAGACGGGCGACAGCTGGTTTCCCTTCCCCCCCCGGGCCTGAGAGAGGTGCAGCAAGCGGt                                                              >  2:25811/1‑139 (MQ=255)
                                                                              tCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGGACCCGGGGGGGTTTTCTTTTTCCCCGTGAGACGGGCAACAGCTGATTTCCCTTCACCCCCTGGCCCTGTGAGAGGTGCAGCAAGCGGTCCACGCTGGgtt                                                  >  2:437808/1‑139 (MQ=255)
                                                                                                          gCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGa                       <  1:196210/138‑1 (MQ=255)
                                                                                                                     aCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAg                                                                  <  1:298219/84‑1 (MQ=255)
                                                                                                                     aCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAg                                                                  >  2:298219/1‑84 (MQ=255)
                                                                                                                      ccAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg          <  2:183228/139‑1 (MQ=255)
                                                                                                                              gtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTTAGAGAGTTGCAGCAAGCGGTCCGCGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGatat  <  1:181213/139‑1 (MQ=255)
                                                                                                                              gtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGata   >  2:440766/1‑138 (MQ=255)
                                                                                                                               |                                                                                                                                         
ATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATAT  >  NZ_CP009273/360635‑360899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑