| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 655,396 | 0 | T | A | 56.2% | 5.4 / 20.7 | 16 | I93F (ATT→TTT) | lipA | lipoyl synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base A (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGAT > NZ_CP009273/655265‑655519 | aaCGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGcc < 2:135531/138‑1 (MQ=255) tAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGCCCAGGGTCATcaa > 1:414779/1‑139 (MQ=255) gcgcCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGAAAAAAAGCGCCGGGGGGCATAAAAAGTGCtgttgcggt > 2:343169/1‑133 (MQ=255) tGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAGGGCCGCGGGTACTAAAAAATGGTGTGCGGTGGGTGAAGCATTTCGccccgg > 1:278272/1‑135 (MQ=255) aTCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAGAGCCGGGGGGCATAAAAAGTGGTGTGCCGGGGGTGGAGCAATTCGCCCGGGTAGGGGCGGAGGGCTTCC‑CGcagac > 2:470099/1‑138 (MQ=255) cTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAAAAGCGCCCAGGATCTCAAACGCTGCTGTGGCGTGGGTGGAGCATTCCCCCAGGGTTGGGCAGGA‑GGGTTTCTCTCAGGCAGaacgtag > 1:235135/1‑134 (MQ=255) ccGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAGCGCCGGGGAGGCTAAAAGGTGGTGTGTCGCGGGTGGAAGATTCCGCCCGGGTAGGGGAGGA‑GGGTTCCTCCCGGGCAGAAAGCAGGCCActttttt > 2:83849/1‑137 (MQ=255) cATCACAGAACGGACAACGGCGGGTACAAAAAGAGCCGAGGATCATAAAAGTTGCTGTGGCGTGGGTGAAGCATTCCGCCCGGGTAGGGGAGGA‑GGGGTTCTCGCAGACAGAAAGCAGGGCCTTTTTTTGCAATGCggg > 1:237748/1‑138 (MQ=255) cATCACAGAACGGACAACGGCGGGGACAAAAAGCCCCGAGGAAGATAAAAATTTGTGTGGCCCGGGTGTAGGACTTCGCCCGGGTAGGGGCGGG‑GGGTTCCTCGCCGACAGAATGCAGGGCAATTTTTCGCCttttggc > 1:462359/1‑134 (MQ=255) cATCACAGAACGGACAACGGCGGGGAAAAAAAACGCCGAGGGGTATAAAAATTGCTGTGGCGGGGTTGAAGGAATCCCCCCGGGTAGGGGAGGG‑GGCGTCCTCGCCGACAGAAAGCAGGCCATTTTTTCGCATTTCGGc > 1:260573/1‑139 (MQ=255) acaGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTg < 2:350910/139‑1 (MQ=255) cGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGccc < 2:237748/139‑1 (MQ=255) cAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGa < 1:83849/139‑1 (MQ=255) cAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGa < 2:189244/139‑1 (MQ=255) aaCGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGAt < 1:470099/139‑1 (MQ=255) aaCGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGAt < 2:462359/139‑1 (MQ=255) | AACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGA‑GGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGAT > NZ_CP009273/655265‑655519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |