| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,844,336 | 0 | T | C | 57.1% | ‑1.8 / 16.5 | 14 | S415P (TCC→CCC) | sppA | signal peptide peptidase SppA |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base C (4/4); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGTTCATACTGAT > NZ_CP009273/1844225‑1844452 | aGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCt > 1:103057/1‑123 (MQ=255)aGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCt < 2:103057/123‑1 (MQ=255) cTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCgg < 2:332539/139‑1 (MQ=255) gCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcacca < 2:245508/139‑1 (MQ=255) tgGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCt > 2:366365/1‑139 (MQ=255) tgGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCTAATTACATTGTGGCTAACCCCAg > 1:79401/1‑82 (MQ=255) tgGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCTAATTACATTGTGGCTAACCCCAg < 2:79401/82‑1 (MQ=255) tgGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCt > 2:189361/1‑139 (MQ=255) tATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTc > 1:420512/1‑139 (MQ=255) tATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGtt > 1:232860/1‑90 (MQ=255) tATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGtt < 2:232860/90‑1 (MQ=255) gATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTCCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGAtt < 2:132712/139‑1 (MQ=255) gcggcatggcggcatCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGgtgt < 1:366365/139‑1 (MQ=255) ggcatCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGTTCATACTGAt < 1:98091/139‑1 (MQ=255) | AGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGTTCATACTGAT > NZ_CP009273/1844225‑1844452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |