| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,145,054 | 0 | A | T | 54.5% | ‑1.8 / 10.4 | 11 | Y130F (TAT→TTT) | yegJ | DUF2314 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base T (6/0); total (8/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGT > NZ_CP009273/2144925‑2145171 | tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGGTTTAACATTGa > 2:123336/1‑139 (MQ=255) gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTAtt < 2:423848/139‑1 (MQ=255) tAATCAGCAATGACCCTGGTATTGTTACAAAAGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTATCCTTGATACTTTTTTTGt > 2:200652/1‑139 (MQ=255) aTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTACCATTTATTCTTTTTTTGTCa > 2:57696/1‑139 (MQ=255) aGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGGTATAACACTTGTCCTTTTTTTGTCACCTCTCCAAAAAAAGAAAACGACGAGCCaa > 1:112576/1‑137 (MQ=255) aCTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTAAACATTTTTCCTTTTTTTGGCCACTATTCAAAAAAAGAAACCGGCGACGTAAGAGGAAAATTAATTTTGTTAACAACCGGTATATTTATTGTTTaaaa > 2:329967/1‑138 (MQ=255) gATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACATTTTTTCTTTTTTTTTGACCCATACAAAAAAAAAAGACGACGAGCGAAGAGGAAAAATAATTCGCTAATCAACCGCTTTATTTTTTTTTTtaaaatgg > 2:448761/1‑134 (MQ=255) cgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg < 1:443879/139‑1 (MQ=255) cgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg < 2:455993/139‑1 (MQ=255) gggATAAAATTTACGGCGGTTTTACCATTTATCCTTTTTTTGTTAACTATACAAAAAAAGAAGCCGGCGAGCTAAAAGCAAAATTAATTTGCTTATCAGACGTTATATTTTTTTTTTTAAATAGCGGGTGGAttttttt > 2:137312/1‑137 (MQ=255) gggATAAAATTTACGGCGGATATTAAAATGTTTCTTTTTTTGTCACCCATCCAAAAAAAAAAACCGACGAGCTAAGAGAAAAAATAGTTTGCTTAACAACCCGTATATTTTTTTTTTTAAATTGCGGGTGGAtttgttt > 1:303970/1‑134 (MQ=255) | TGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGT > NZ_CP009273/2144925‑2145171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |