| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,342,624 | 0 | A | C | 58.3% | 4.6 / 13.1 | 12 | F110V (TTC→GTC) | inaA | lipopolysaccharide kinase InaA |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTC > NZ_CP009273/2342486‑2342747 | cATAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGaa > 1:96101/1‑139 (MQ=255) ccATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCatat > 1:239612/1‑139 (MQ=255) cTGACGATTAATGCTATGCATTTTCTTAACCGCCAGCGCCACTGCTTTCAACATGGCTTGCCGTTCTTCGTCAGAATAAGGCGATACTGCATGCGGGGCATACCAGCCAGCAATGTTGATGACCCCCGCCATATCTTCa < 1:319638/139‑1 (MQ=255) tCTTAACCGCCAGCGCCACTGTTTTCAACATGGCTGGCCGTACTTCGTCAGAAAAAGGCGATCCTGCATGCTGGGCATCCCAGTCAGCAATGCTAATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCAtt < 1:485088/138‑1 (MQ=255) tCTTAAACGCCAGCGCCACTGCTTTCAACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCAtt > 1:332140/1‑138 (MQ=255) aaCCCCAGCGCCCCTCCTTCAAACGTGGCTGGCCGTACTTCGTAAAAAAAAGGCGATACGGCAGGCGGGGCAACCCAGACAGAAATGCTGAGAACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCt < 2:230805/139‑1 (MQ=255) gcgcCACTGCTTTCAACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAAtttt > 1:278507/1‑139 (MQ=255) cTGCTTTCAACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGc > 1:406979/1‑139 (MQ=255) gCCGTACCTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTAAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAATGATCtt > 2:37138/1‑139 (MQ=255) gTACTTCGTCAGAAAAAGGCGATCCTGCTTGCGGGCCATACCAGCCAGCATTGCTGATGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCgg < 2:406979/139‑1 (MQ=255) aGGCAATCCTGCATGCTGGGCATCCCAGTCAGAAATGCTGATAACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGcc < 1:287663/139‑1 (MQ=255) cATCCTGGCCATACAAGTCAATAATCCTAGTAACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTc < 1:236917/139‑1 (MQ=255) cAGCAATGCTGACGACCCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTc < 2:96101/139‑1 (MQ=255) | CATAACAACAGCCATGCTGACGATTAATGCTATGCATTTTCTTAAACGCCAGCGCCACTGCTTTCAACATGGCTTGCCGTACTTCGTCAGAATAAGGCGATACTGCATGCTGGGCATACCAGTCAGCAATGCTGATGAACCCCGCCATATCTTCAGTCACCAGCAACGCTCGCCATTCACCCTCAATTTTCACCGCTTCACCAAAAACGATCTTCGGCACGATGACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTC > NZ_CP009273/2342486‑2342747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |