| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,406,265 | 0 | T | A | 56.2% | ‑1.6 / 17.0 | 16 | Y187F (TAT→TTT) | acrS | multidrug efflux transporter transcriptional repressor AcrS |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base A (0/9); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGC > NZ_CP009273/3406131‑3406389 | aCGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTtata > 1:365837/1‑136 (MQ=255) ttACATGACTTTTAATTCATTCGTTTGATAAATTATTTTCTTTATTTTTTCATCTGGCATAAACATTTTAAATCCGTTACCCCCCAGAGGGGGGGTTTTTTAAAAAAGATCATACCCCGCCATATTCATTAACCAGtt < 2:458435/138‑1 (MQ=255) tACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGtttt > 2:458298/1‑139 (MQ=255) tACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGtttt > 2:117282/1‑139 (MQ=255) gTTTGATAAATTAATTTCTTTTTTTTTTCATCTGGCATAAACATTTTTAATCCTTTACCGACCAGGGGGGGGGTTTGTTTAAAAAGATAAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCa < 2:234550/138‑1 (MQ=255) gTTTAATAAATTAATTTCTTTTTTTTTTCATCTGGCATAAACATTTTTAATACTTTATCGACCAGAGCGGGGGTTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCa < 1:146578/138‑1 (MQ=255) attaattTCGTTATGTTTTCATTTGGCAGGAACATTATTAATCCGTTGCCGACCGGGGCGGGGGTTTTTTTAAAAAGATCAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaata < 2:325625/138‑1 (MQ=255) gTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAAtcatca > 2:237626/1‑139 (MQ=255) tATTTTTTCATCTGGCATGAACATTCTTAATACTTTATCGCCCAGAGCGGGGGTTTGTTTAAAAAGATCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATcac < 2:97764/138‑1 (MQ=255) aaaaaaTTTTTAATCCGTTAGCGACCAGAGGGGGGTTTTTTTAAAAAAAATAAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGtt < 2:136374/134‑1 (MQ=255) aaCATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt > 1:104538/1‑139 (MQ=255) aCATTCTTAATCCTTTACCGACAAGGGCGGGGTTTTTTTTAAAAAAACCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTAtt < 1:242889/139‑1 (MQ=255) aTTTTTAATCCGTTACCGCCCAGAGCGGGGGTTTGTTTAAAAAAATTAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTg < 2:324958/139‑1 (MQ=255) tttaaaTCCGTTATCGACCAGAGCGGGGGTTTTTTTAAAAAAATCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTg < 2:104538/133‑1 (MQ=255) ttATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCtt > 1:143422/1‑139 (MQ=255) ccaaCCAGAGGGGGGGTTTTTTTAAAAAAATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttgtt < 1:458298/136‑1 (MQ=255) gggggtCTTGTTTATAAAGAAAAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAAGCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGc < 2:143422/138‑1 (MQ=255) | ACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGC > NZ_CP009273/3406131‑3406389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |